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Series GSE47696 Query DataSets for GSE47696
Status Public on Jul 01, 2017
Title Cyclodextrin treatment leads to atherosclerosis regression by dissolution of cholesterol crystals and LXR activation
Organism Mus musculus
Experiment type Expression profiling by array
Summary Atherosclerosis is an inflammatory disease linked to elevated blood cholesterol levels. Since cholesterol retention and cholesterol crystals in arterial walls are key pathogenetic factors for atherogenesis, we assessed the therapeutic potential of increasing cholesterol solubility in vivo. Here we show that treatment of murine atherosclerosis with the cyclic oligosaccharide 2-hydroxypropyl-β-cyclodextrin (CD), a compound that solubilizes lipophilic substances, reduced atherosclerotic plaque size, cholesterol crystal (CC) load and promoted plaque regression even under continuing Western diet. CD solubilized CC and promoted cholesterylester and oxysterol production in macrophages leading to liver X receptor-mediated transcriptional reprogramming with increased cholesterol efflux and decreased inflammation. CD treatment may thus be used to increase cholesterol solubility and clearance to prevent or treat atherosclerosis.
 
Overall design Bone marrow-derived macrophages (BMDMs) were derived from bone marrow isolated from tibiae and femurs of LXR -/- -/- mice on a C57BL/6 background and age matched littermate wild type mice. Bone marrow cells were cultured in DMEM supplemented with 10 % FCS, 10 g/ ml Ciprobay-500 and 40 ng/ ml M-CSF (R&D Systems) for six days. BMDMs were treated for 3h with 200 ug cholesterol crystals per 1 mio. cells or control medium. Subsequently cells were incubated for 6h with 10 mM CD before cell lysis in Trizol (Invitrogen). Samples were stored at -80 C for RNA isolation.
 
Contributor(s) Zimmer S, Grebe A, Bode N, De Nardo D, Labzin L, Hempel C, Kerksiek T, Heneka M, Ulas T, Schultze J, Nickenig G, Lütjohann D, Latz E
Citation(s) 27053774
Submission date Jun 06, 2013
Last update date Jan 16, 2019
Contact name Joachim Schultze
E-mail(s) j.schultze@uni-bonn.de
Organization name LIMES (Life and Medical Sciences Center Genomics and Immunoregulation)
Department Genomics and Immunoregulation
Street address Carl-Troll-Strasse 31
City Bonn
State/province NRW
ZIP/Postal code 53115
Country Germany
 
Platforms (1)
GPL6887 Illumina MouseWG-6 v2.0 expression beadchip
Samples (20)
GSM1155046 CD-cc_replicate 1
GSM1155047 CD-cc_replicate 2
GSM1155048 CD-cc_replicate 3
Relations
BioProject PRJNA207331

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE47696_RAW.tar 15.8 Mb (http)(custom) TAR
GSE47696_non-normalized_data.txt.gz 5.6 Mb (ftp)(http) TXT
Processed data included within Sample table

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