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Series GSE47734 Query DataSets for GSE47734
Status Public on Feb 12, 2014
Title Interplay of host microbiota, genetic perturbations, and inflammation promotes local development of intestinal neoplasms in mice [BeadArray]
Organism Mus musculus
Experiment type Expression profiling by array
Summary The preferential localization of some neoplasms, such as serrated polyps, in specific areas of the intestine suggests that non-genetic factors may be important for their development. To test this hypothesis, we took advantage of transgenic mice that expressed HB-EGF throughout the intestine, but develop serrated polyps only in the cecum.
Here we show that a host-specific microbiome was associated with serrated polyps, and that alterations of the microbiota induced by antibiotic treatment or by embryo-transfer rederivation markedly inhibited the formation of serrated polyps in the cecum. Mechanistically, development of serrated polyps was associated with a local decrease in epithelial barrier-function, bacterial invasion, production of antimicrobials, and increased expression of several inflammatory factors such as IL-17, Cxcl2, Tnf-α, and IL-1. Increased number of neutrophils were found within the serrated polyps, and their depletion significantly reduced polyp growth.
Together these results indicate that non-genetic factors contribute to the development of serrated polyps and suggest that the development of these intestinal neoplasms in the cecum is driven by the interplay between genetic changes in the host, an inflammatory response, and a host-specific microbiota.
 
Overall design Paired design; Surrounding and SP samples were obtained from the same mouse (n=3; mouse 1, 2, 3)
 
Contributor(s) Bongers G, Pacer ME, Geraldino TH, Chen L, He Z, Hashimoto D, Furtado GC, Ochando J, Kelley KA, Clemente JC, Merad M, van Bakel H, Lira SA
Citation(s) 24590763
Submission date Jun 07, 2013
Last update date Jun 14, 2018
Contact name Gerold Bongers
E-mail(s) gerold.bongers@mssm.edu
Organization name Icahn School of Medicine at Mount Sinai
Department Immunology Institute
Street address 1425 Madison Ave, 12-26A
City New York
State/province NY
ZIP/Postal code 10029-6574
Country USA
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (6)
GSM1155636 Mouse 1, SP
GSM1155637 Mouse 1, Surrounding
GSM1155638 Mouse 2, SP
This SubSeries is part of SuperSeries:
GSE47736 Interplay of host microbiota, genetic perturbations, and inflammation promotes local development of intestinal neoplasms in mice
Relations
BioProject PRJNA207542

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE47734_RAW.tar 3.1 Mb (http)(custom) TAR
GSE47734_non-normalized.txt.gz 544.8 Kb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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