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Status |
Public on Nov 01, 2013 |
Title |
Pregnancy-associated genes contribute to antiluteolytic mechanisms in ovine coprus luteum |
Platform organism |
Bos taurus |
Sample organism |
Ovis aries |
Experiment type |
Expression profiling by array
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Summary |
Ovine interferon-tau (IFNT) is released from the conceptus by Day 12 of pregnancy and disrupts pulsatile release of endometrial prostaglandin F2 alpha (PGF), thereby protecting the corpus luteum (CL). IFNT may also have endocrine action through inducing interferon stimulated genes (ISGs) in the CL. The hypothesis that gene expression differs in CL collected from pregnant (P) and non-pregnant (NP) ewes by Day 14 due to the lytic action of PGF during the estrous cycle or the presence of a conceptus was tested. RNA was isolated on Days 12 and 14 in NP or P ewes (n = 3 ewes/group) and analyzed using the Affymetrix bovine microarray (24,000 targets). Differential gene expression (>1.5 fold, P < 0.05) was confirmed using semi-quantitative real time PCR (RTPCR). Serum progesterone concentrations decreased (P < 0.05) from 1.7 ng/ml on Day 12 to 1.3 ng/ml by Day 14 in NP ewes suggesting initiation of luteolysis; and remained > 1.7 ng/ml in Day 12 and 14 P ewes indicating that the conceptus protected the CL from luteolysis. Early luteolysis from Day 12 to 14 NP was associated with differential expression of 683 genes, including SERPINE1 and THBS1. Presence of a conceptus from Day 12 to 14 also induced expression of 743 genes, i.e., ISGs (ISG15, MX1), PTX3, and IL-6 and stabilized expression of VEGF and LHR genes. In conclusion, pregnancy circumvents luteolytic pathways, and activates or stabilizes genes associated with interferon, chemokine, cell adhesion, cytoskeletal, angiogenic and epithelial to mesynchymal transition pathways in the CL.
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Overall design |
There are 12 samples that were analyzed for the microarray, no duplicates and we increased the sample size to 50 for the RTPCR. This Series contains the Affymetrix array data only (not RT-PCR data).
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Contributor(s) |
Romero JJ, Antoniazzi AQ, Smirnova NP, Webb BT, Yu F, Davis JS, Hansen TR |
Citation missing |
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Submission date |
Jun 10, 2013 |
Last update date |
Nov 03, 2013 |
Contact name |
Thomas Hansen |
E-mail(s) |
thomas.hansen@colostate.edu
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Phone |
9704918081
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Organization name |
Colorado State University
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Department |
Biomedical Sciences
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Street address |
3107 Rampart Rd
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City |
Fort Collins |
State/province |
CO |
ZIP/Postal code |
80523 |
Country |
USA |
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Platforms (1) |
GPL2112 |
[Bovine] Affymetrix Bovine Genome Array |
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Samples (12)
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Relations |
BioProject |
PRJNA207799 |
Supplementary file |
Size |
Download |
File type/resource |
GSE47776_RAW.tar |
24.6 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
Processed data included within Sample table |
Processed data provided as supplementary file |
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