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Series GSE4786 Query DataSets for GSE4786
Status Public on May 01, 2007
Title Caloric restriction suppresses apoptotic cell death in the mammalian cochlea and leads to prevention of presbycusis
Organism Mus musculus
Experiment type Expression profiling by array
Summary Presbycusis is characterized by an age-related progressive decline of auditory function, and arises mainly from the degeneration of hair cells or spiral ganglion (SG) cells in the cochlea. Here we show that caloric restriction suppresses apoptotic cell death in the mouse cochlea and prevents late onset of presbycusis. Caloric restricted mice, which maintained body weight at the same level as that of young control (YC) mice, retained normal hearing and showed no cochlear degeneration. CR mice also showed significantly fewer TUNEL-positive staining cells and fewer cleaved caspase-3-positive staining cells relative to middle-age control (MC) mice. Microarray analysis revealed that CR down-regulated the expression of 28 proapoptotic genes, including Bak and Bim. Taken together, our findings suggest that loss of critical cells through apoptosis is an important mechanism of presbycusis in mammals, and that CR or staying lean can retard this process by suppressing apoptosis in the inner ear tissue.
Keywords: Effect of aging, effect of caloric restriction, time course, disease state analysis
 
Overall design To examine the effects of aging, a comparison of cochlea tissues from YC (3 samples) and MC (3 samples) mice was conducted. To examine the effects of calorie restriction (CR), a comparison of cochleae from MC (3 samples) and CR (3 samples) mice was conducted. We examined age-related changes in gene expression in the cochlea and calorie restriction-induced changes in gene expression in the cochlea. We pooled four cochleae from two mice for one sample and used three samples per group (n = 3).
Quality control measures were not used. No replicates were done. Dye swap was not used.
 
Contributor(s) Someya S, Yamasoba T, Weindruch R, Prolla TA, Tanokura M
Citation(s) 16890326
Submission date May 06, 2006
Last update date Feb 11, 2019
Contact name Shinichi Someya
E-mail(s) someya@wisc.edu
Phone 608-265-5205
Fax 608-262-2976
URL http://www.genetics.wisc.edu/faculty/profile.php?id=140
Organization name University of Wisconsin-Madison
Department Medical Genetics
Lab Prolla Lab
Street address 425 Henry Mall Dr
City Madison
State/province WI
ZIP/Postal code 53706
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (9)
GSM108100 Cochlea_CR_15mo_1
GSM108101 Cochlea_CR_15mo_2
GSM108102 Cochlea_CR_15mo_3
Relations
BioProject PRJNA95543

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE4786_RAW.tar 51.7 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file

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