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Series GSE4799 Query DataSets for GSE4799
Status Public on May 11, 2006
Title GDNF-regulated gene expression in cultured mouse spermatogonial stem cells
Organism Mus musculus
Experiment type Expression profiling by array
Summary GDNF-regulated gene expression was studied in cultures of actively self-renewing spermatogonial stem cells established from 6 day old male mice. GDNF is the essential growth factor regulating mouse spermatogonial stem cell self-renewal. Using a serum-free chemically defined culture system that supports mouse spermatogonial stem cell self-renewal for extended periods of time, GDNF-regulated genes were identified using microarray profiling.
Keywords: GDNF withdrawal and time-course replacement
Overall design Established cultures of highly enriched self-renewing spermatogonial stem cells were subjeted to withrawal of GDNF and GFRalpha1 for 18-hr followed by replacement of the growth factors for 2, 4, and 8-hr. Gene expression was studied using microarray profiling prior to withdrawal, after withdrawal and at each time-point of GDNF/GFRalpha1 replacement.
Contributor(s) Oatley JM, Avarbock MR, Telaranta AI, Fearon DT, Brinster RL
Citation(s) 16740658
Submission date May 09, 2006
Last update date Feb 11, 2019
Contact name Jon Oatley
Organization name Pennsylvania State University
Department Dairy and Animal Science
Street address 324 Henning
City University Park
State/province PA
ZIP/Postal code 16802
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (15)
GSM108310 SSC GDNF/GFRalpha1 Rep1
GSM108311 SSC GDNF/GFRalpha1 Rep2
GSM108312 SSC GDNF/GFRalpha1 Rep3
BioProject PRJNA95745

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