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Series GSE48043 Query DataSets for GSE48043
Status Public on Jul 01, 2013
Title Late life rapamycin treatment reverses age-related heart dysfunction
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We report the mRNA profile of aged mice (24 months old) fed either a control diet or a diet containing Rapamycin (14 ppm) for 3 months. After drug treatement, the hearts of the mice were removed and total mRNA was removed from the tissue. Analysis revealed that there were 700 significantly differentially expressed genes between the control fed group and the Rapamycin diet group by our analysis.
 
Overall design Heart tissue samples from age-matched control mice (n=10) and rapamycin fed mice (n=10) were extracted for total RNA. The samples were sequenced using Illumina HiSeq 2000 (50 basepair paired-end sequencing). The sequencing yielded quality scores greater than 30 with an average of 10 million reads per sample. 34,293 genes were mapped back to the MGSCv37 C57BL/6J mouse genome (maximum paired distance=300 and minimum=130, minimum number of reads per mapping = 5, maximum number of mismatches= 2, with the reads being mapped to unique sites in the genome).
 
Contributor(s) Flynn JM, O’Leary MN, Zambataro CA, Academia EC, Presley MP, Garrett BJ, Zykovich A, Mooney SD, Strong R, Rosen CJ, Nelson MD, Kapahi P, Kennedy BK, Melov S
Citation(s) 23734717
Submission date Jun 17, 2013
Last update date May 15, 2019
Contact name Simon Melov
E-mail(s) smelov@buckinstitute.org
Phone 415 209 2068
Organization name Buck Institute for Research on Aging
Lab Melov Lab
Street address 8001 Redwood Blvd
City Novato
State/province CA
ZIP/Postal code 94945
Country USA
 
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (20)
GSM1166231 Rapa_S3
GSM1166232 Rapa_S15
GSM1166233 Rapa_S5
Relations
BioProject PRJNA208747
SRA SRP026129

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE48043_All_genes.txt.gz 651.7 Kb (ftp)(http) TXT
GSE48043_DE_genes_processed.txt.gz 75.4 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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