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Series GSE48128 Query DataSets for GSE48128
Status Public on Jul 01, 2013
Title Regulation of transcription by MLL2 complex and MLL complexes-associated protein AKAP95
Organism Mus musculus
Experiment type Expression profiling by array
Summary Although histone H3 lysine 4 (H3K4) methylation is widely associated with gene activation, direct evidence for its causal role in transcription, through specific MLL family members, is scarce. Here we have purified a human MLL2 (Kmt2b) complex that is highly active in H3K4 methylation and chromatin transcription in a cell-free system. This effect requires SAM and intact H3K4, establishing a direct and causal role for MLL2-mediated H3K4 methylation in transcription. We then show that human AKAP95, a chromatin-associated protein, is physically and functionally associated with the Dpy-30-MLL complexes and directly enhances their methyltransferase activity. Ectopic AKAP95 stimulates expression of a chromosomal reporter in synergy with MLL1 or MLL2, whereas AKAP95 depletion impairs retinoic acid-mediated gene induction in embryonic stem cells. These results demonstrate an important role for AKAP95 in regulating histone methylation and gene expression, particularly during cell fate transitions.
 
Overall design Total RNAs from control or knockdown cells before and after RA-mediated differentiation were subjected to Illumina microarray analyses.
The complete dataset containing non_normalized, median-normalized and expression ratio (relative to Scramble shRNA, undifferentiated data) is linked below as a supplementary file [complete_data.txt].
 
Contributor(s) Jiang H, Roeder RG
Citation(s) 23995757
Submission date Jun 19, 2013
Last update date Jun 14, 2018
Contact name Hao Jiang
E-mail(s) haojiang@uab.edu
Organization name University of Alabama at Birmingham
Department Biochemistry and Molecular Genetics
Street address 1825 University Blvd
City Birmingham
State/province AL
ZIP/Postal code 35294
Country USA
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (4)
GSM1169161 Scramble shRNA, RA-differentiated
GSM1169162 AKAP95 shRNA, RA-differentiated
GSM1169163 Scramble shRNA, undifferentiated
Relations
BioProject PRJNA208969

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE48128_RAW.tar 3.1 Mb (http)(custom) TAR
GSE48128_complete_data.txt.gz 2.8 Mb (ftp)(http) TXT
GSE48128_non_normalized.txt.gz 747.6 Kb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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