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| Status |
Public on Oct 21, 2013 |
| Title |
Identification of miRNA targets in breast cancer cells (Ago2-IP) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
miRNAs regulate mRNA stability and translation through the action of the RNAi-induced silencing complex. In this study, we systematically identified endogenous miRNA target genes by using AGO2 immunoprecipitation (AGO2-IP) and microarray analyses in two breast cancer cell lines, MCF7 and MDA-MB-231, representing luminal and basal-like breast cancer, respectively. The expression levels of ~70% of the AGO2-IP mRNAs were increased by DROSHA or DICER1 knockdown. In addition, integrated analysis of miRNA expression profiles, mRNA-AGO2 interaction, and the 3'-UTR of mRNAs revealed that >60% of the AGO2-IP mRNAs were putative targets of the fifty most abundantly expressed miRNAs.
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| Overall design |
To identify mRNAs associated with AGO2, cell lysate was precleaned with control IgG and immunoprecipitated with anti-human Ago2 (Clone 2E12-1C9, Abnova). Total RNA from cell lysate or coimmunoprecipitated with AGO2 was extracted with Trizol and subjected to microarray analysis, with three biological repeats for each experimental condition.
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| Contributor(s) |
Fan M, Krutilina R, Sun J, Sethuraman A, Yang CH, Wu Z, Yue J, Pfeffer LM |
| Citation(s) |
23921383 |
| Submission date |
Jun 20, 2013 |
| Last update date |
Aug 16, 2018 |
| Contact name |
Meiyun Fan |
| E-mail(s) |
mfan2@uthsc.edu
|
| Organization name |
University of Tennessee
|
| Street address |
19 S. Manassas Street
|
| City |
Memphis |
| ZIP/Postal code |
38163 |
| Country |
USA |
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| Platforms (1) |
| GPL6947 |
Illumina HumanHT-12 V3.0 expression beadchip |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE48162 |
Identification of miRNA targets in breast cancer cells |
|
| Relations |
| BioProject |
PRJNA209062 |
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