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| Status |
Public on Sep 01, 2013 |
| Title |
Molecular Characterization of Heat Intolerance in Mice (mRNA data) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Prolonged exposure to high temperatures may cause heat-related illnesses, such as cramps, syncope, exhaustion or even stroke in some individuals. Heat-related injuries remain a threat to the health and operational effectiveness of military personnel, athletes and the general public. Heat injury victims experience long-term complications that may include multi-system organ (liver, kidney, muscle) and neurologic damage, as well as reduced exercise capacity and heat intolerance. Findings from our laboratory using a developed heat stress model show that about 1/3 of mice are heat-intolerant and vulnerable to heat injury even though they are from the same mice litter.
We examined if there is any genetic causation to this pattern of observation between the two groups of mice classified (Heat Intolerant and Heat Tolerant). We would like to screen Heat Tolerant and Heat Intolerant mice samples using microarray technology and examine their microRNA and mRNA for possible gene-specific differences between the two groups (6 mice per group). The results from this proposed animal research will help identify and select potential markers that can be used as a pre-screen to identify heat intolerance and assess heat injury recovery in humans.
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| Overall design |
Heat-induced physiological and biochemical changes were assessed to determine heat tolerance levels in mice. We performed mRNA and microRNA expression profiling on mouse gastrocnemius muscle tissue samples to determine novel biological pathways associated with heat tolerance.
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| Contributor(s) |
Ghimbovschi S, Islam A |
| Citation(s) |
23967293 |
| Submission date |
Jun 25, 2013 |
| Last update date |
Feb 07, 2020 |
| Contact name |
Svetlana Ghimbovschi |
| Organization name |
Children's National Medical Center
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| Department |
Research Center for Genetic Medicine
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| Lab |
Dr. Hoffman's Lab.
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| Street address |
111 Michigan Ave., NW
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| City |
Washington |
| State/province |
DC |
| ZIP/Postal code |
20010 |
| Country |
USA |
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| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
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| Samples (12)
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| GSM1173830 |
Heat_Intolerant_Mouse-3, biological rep1 |
| GSM1173831 |
Heat_Intolerant_Mouse-4, biological rep2 |
| GSM1173832 |
Heat_Intolerant_Mouse-5, biological rep3 |
| GSM1173833 |
Heat_Intolerant_Mouse-7, biological rep4 |
| GSM1173834 |
Heat_Intolerant_Mouse-9, biological rep5 |
| GSM1173835 |
Heat_Intolerant_Mouse-11, biological rep6 |
| GSM1173836 |
Heat_Tolerant_Mouse-3, biological rep7 |
| GSM1173837 |
Heat_Tolerant_Mouse-4, biological rep8 |
| GSM1173838 |
Heat_Tolerant_Mouse-5, biological rep9 |
| GSM1173839 |
Heat_Tolerant_Mouse-7, biological rep10 |
| GSM1173840 |
Heat_Tolerant_Mouse-9, biological rep11 |
| GSM1173841 |
Heat_Tolerant_Mouse-11, biological rep12 |
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| This SubSeries is part of SuperSeries: |
| GSE48271 |
Molecular Characterization of Heat Intolerance in Mice: microRNA (Affymetrix) and mRNA (Illumina) platforms |
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| Relations |
| BioProject |
PRJNA209473 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE48269_RAW.tar |
15.8 Mb |
(http)(custom) |
TAR |
| GSE48269_non-normalized_matrix.txt.gz |
2.1 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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