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Series GSE48365 Query DataSets for GSE48365
Status Public on Jun 27, 2013
Title S-adenosylmethionine modifies cocaine-induced DNA methylation and increases locomotor sensitization in mice
Organism Mus musculus
Experiment type Expression profiling by array
Summary The aim of the study was to investigate whether environmental factors like S-adenosylmethionine (SAM) via affecting epigenome could alter cocaine-induced gene expression and locomotor sensitization in mice.
Using mouse nucleus accumbens (NAc) tissue, whole-genome gene expression profiling revealed that repeated SAM treatment affected a limited number of genes, but significantly modified cocaine-induced gene expression by blunting nonspecifically the cocaine response.
At the gene level, we discovered that SAM modulated cocaine-induced DNA methylation by inhibiting both promoter-associated CpG-island hyper- and hypomethylation in the NAc but not in the reference tissue cerebellum.
 
Overall design Total RNA was extracted from the mouse nucleus accumbens (NAc) tissue. Two tissues were combined to a sample, 4 samples per group used. RNA quality and quantity were assessed using the Nano-Drop -1000 spectrophotometer and the Agilent 2100 Bioanalyzer.
 
Contributor(s) Anier K, Zharkovsky A, Kalda A
Citation(s) 23684129
Submission date Jun 27, 2013
Last update date Jun 14, 2018
Contact name Kaili Anier
E-mail(s) kaili.anier@gmail.com
Organization name University of Tartu
Street address Ravila 19
City Tartu
ZIP/Postal code 50411
Country Estonia
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (16)
GSM1176475 Saline + Saline (S+S) 1
GSM1176476 Saline + Saline (S+S) 2
GSM1176477 Saline + Saline (S+S) 3
Relations
BioProject PRJNA209848

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE48365_RAW.tar 3.1 Mb (http)(custom) TAR
GSE48365_non-normalized_data.txt.gz 3.2 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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