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Series GSE4847 Query DataSets for GSE4847
Status Public on Dec 29, 2006
Title Expression data from tocopherol deficient seedlings of Arabidopsis
Organism Arabidopsis thaliana
Experiment type Expression profiling by array
Summary Tocopherols (Vitamin E) are lipophilic antioxidants that are synthesized by all plants and are particularly abundant in seeds. Two tocopherol deficient mutant loci were used to examine how tocopherol deficiency impacts global gene expression during the critical peroid of germination and early seedling development when tocopherols are essential. vte1 lacks all tocopherols, but accumulates the tocopherol pathway intermediate DMPBQ,. vte2 which lacks all tocopherols and pathway intermediates.
We used microarrays to examine the global gene expression in early seedlings and identify distinct classes of genes whose expression is affected by tocopherol deficient mutants vte1 and vte2.
Keywords: genetic modification
 
Overall design Arabidopsis seedlings were grown on media containing MS salts at 22C and RNA was extracted from 1 (1D)and 3 (3D)day old seedlings following cold treatment to break dormancy and synchronize germination (5 days at 6C) . We sought to compare the two tocopherol deficient mutants vte1 (VTE1) and vte2 (VTE2) with each other and the corresponding wild-type background Columbia-0 (COL).
 
Contributor(s) Sattler SE, DellaPenna D
Citation(s) 17194769
Submission date May 15, 2006
Last update date Aug 28, 2018
Contact name Scott E. Sattler
E-mail(s) sattler4@msu.edu
Phone 517-432-9283
Fax 517-353-9334
Organization name Michigan State University
Department Biochemistry and Molecular Boliogy
Lab Dean DellaPenna
Street address room 201 Biochem Bldg
City East Lansing
State/province MI
ZIP/Postal code 48824
Country USA
 
Platforms (1)
GPL198 [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array
Samples (18)
GSM109107 wild-type(Col-0) 1 day, biological rep1
GSM109108 wild-type(Col-0) 1 day, biological rep2
GSM109109 wild-type(Col-0) 1 day, biological rep3
Relations
Affiliated with GSE69995
BioProject PRJNA95845

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Supplementary file Size Download File type/resource
GSE4847_RAW.tar 53.9 Mb (http)(custom) TAR (of CEL)

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