|
| Status |
Public on Dec 25, 2013 |
| Title |
ChIP-seq in MRC5 hIPSCs overexpressing MEG3 RNA. |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
This experiment was designed to detect differences in PRC2 occupancy in a human induced pluripotent cell line (MRC5) naturally deficient in MEG3 RNA after lentiviral overexpression of MEG3.
|
| |
|
| Overall design |
MRC5 were transduced with lentiviruses for the overexpression of MEG3 lncRNA or GFP as a control. ChIP-seq was performed with EZH2 and JARID2 antibodies.
|
| |
|
| Contributor(s) |
Kaneko S, Bonasio R, Reinberg D |
| Citation(s) |
24374312 |
| Submission date |
Jul 03, 2013 |
| Last update date |
Feb 04, 2022 |
| Contact name |
Roberto Bonasio |
| Organization name |
University of Pennsylvania
|
| Department |
Cell and Developmental Biology
|
| Lab |
Bonasio
|
| Street address |
3400 Civic Center Blvd - SCTR 9-111
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (6)
|
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| This SubSeries is part of SuperSeries: |
| GSE48518 |
Interactions between JARID2 and noncoding RNAs regulate PRC2 recruitment to chromatin |
|
| Relations |
| BioProject |
PRJNA210418 |
| SRA |
SRP026532 |
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