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Series GSE48758 Query DataSets for GSE48758
Status Public on Oct 01, 2014
Title Targeted disruption of BMAL1 in the peripheral reproductive axis results in infertility in female mice
Organism Mus musculus
Experiment type Expression profiling by array
Summary The product of the Bmal1 locus is an essential component of the circadian clock that plays important roles in various aspects of reproductive biology,and when disrupted results in infertility. In an effort to establish the identity of the tissue specific clock that is responsible for this infertility, we used the steroidogenic factor-1 (Sf1) promoter to drive Cre-mediated recombination and genetically delete Bmal1 within cells of the reproductive axis. We show that Bmal1 within the reproductive axis of females is essential for normal fertility through its role in maintaining implantation, but is not required for normal estrous cycling. At the root of this biology appears to be a defect in the ovaries, including regulation of ovarian lipid biosynthetic or metabolic processes and their roles in maintaining progesterone synthesis. This conclusion is based upon three observations. First, that deletion of Bmal1 within the reproductive axis reducesleads to affected transcripts in steroidogenic pathways for the LH receptor , and lowers progesterone levels. Second, that progesterone supplementation of these conditional mutants rescues implantation. Third, transplantation of wild type ovaries into Bmal1 reproductive axis mutants rescues fertility. Our study demonstrates the significance of ovarian Bmal1 as an overriding influence in experimental models of infertility.
 
Overall design A time series was performed in time-mated C57Bl/6J mice to identiy oscillating transcripts.
During the peak and trough of the majority of transcripts (ZT0 and ZT12) samples from Bmal1fx/fx Sf1Cre mice and control litermates as well and global Bmal1 nulls were also analyzed.
The tissue types (ovary, pituitary) are not comparable.
 
Contributor(s) Johnson BP, Liu Y, Bradfield CA
Citation(s) 25225411
Submission date Jul 10, 2013
Last update date Feb 02, 2018
Contact name Brian P Johnson
Phone 608-262-1209
Organization name UW-Madison
Department Oncology
Lab Chris Bradfield
Street address 1400 University Ave
City Madison
State/province WI
ZIP/Postal code 53706
Country USA
 
Platforms (1)
GPL10787 Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Probe Name version)
Samples (56)
GSM1183863 C57Bl/6J Ovary 66hours post conception (ZT12)_1 2-5dpc ZT12 WT ovary
GSM1183864 C57Bl/6J Ovary 70hours post conception (ZT16)_2 2-5dpc ZT16 WT ovary
GSM1183865 C57Bl/6J Ovary 74hours post conception (ZT20)_3 2-5dpc ZT20 WT ovary
Relations
BioProject PRJNA211526

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE48758_RAW.tar 677.4 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table
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