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| Status |
Public on May 24, 2014 |
| Title |
Expression data of mouse developmental T cells (CD4CD8DP, CD4SP and CD8SP) derived from Brd1 deficient thymocyte |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Conditional knock out of Brd1(bromodomain containing 1) in muse developmental T cells (CD4CD8DP, CD4SP and CD8SP) by intercrossing with Tie2-Cre
Conditional knock out of Brd1(bromodomain containing 1) in muse MHC class l restricted T cells (CD8SP) by intercrossing with Tie2-Cre and OT-l
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| Overall design |
Developmental T cells (defined by surface markers: CD4, CD8 and TCRβ) derived from control (Tie2-Cre) or Brd1 conditional knock out (Brd1flox/flox, Tie2-Cre) thymocytes were isolated by fluorescence activated cell sorting (FACS) and subjected to a microarray analysis. CD8SP T cells were also isolated from control (Tie2-Cre, OT-l) or Brd1 conditional knock out (Brd1flox/flox, Tie2-Cre, OT-l) thymocytes intercrossed with OT-l.
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| Contributor(s) |
Mishima Y, Iwama A, Miyagi S |
| Citation(s) |
25519988 |
| Submission date |
Jul 11, 2013 |
| Last update date |
Jun 10, 2019 |
| Contact name |
ATSUSHI IWAMA |
| E-mail(s) |
aiwama@faculty.chiba-u.jp
|
| Phone |
81-43-226-2187
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| Fax |
81-43-226-2191
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| Organization name |
Graduate School of Medicine, Chiba University
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| Department |
Department of Cellular and Molecular Medicine
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| Street address |
1-8-1 Inohana, Chuo-ku
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| City |
Chiba |
| ZIP/Postal code |
260-8670 |
| Country |
Japan |
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| Platforms (1) |
| GPL10787 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Probe Name version) |
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| Samples (9)
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| GSM1184699 |
CD4+CD8+DP cells derived from control 9-1-WD |
| GSM1184700 |
CD4SP cells derived from control 10-2-W4 |
| GSM1184701 |
CD8SP cells derived from control 11-3-W8 |
| GSM1184702 |
CD4+CD8+DP cells derived from Brd1 CKO 3-4-FD2 |
| GSM1184703 |
CD4SP cells derived from Brd1 CKO 4-5-4P2 |
| GSM1184704 |
CD4SP(TCRβ-) cells derived from Brd1 CKO 5-6-F4N2 |
| GSM1184705 |
CD8SP cells derived from Brd1 CKO 6-7-F82 |
| GSM1184706 |
CD8SP cells derived from control OT-l 16-8-W8O |
| GSM1184707 |
CD8SP cells derived from Brd1 CKO OT-l 17-9-F8O |
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| Relations |
| BioProject |
PRJNA211803 |
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