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| Status |
Public on Jul 13, 2013 |
| Title |
Structural basis of a novel Interferon b signaling axis mediated via the ifnar1 receptor |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Type I interferons (IFNs) are a family of cytokines that play an important role in regulating immune responses to pathogens and tumors and are used therapeutically. All IFNs are considered to signal via the heterodimeric IFNAR1-IFNAR2 complex, yet some subtypes such as IFN? can exhibit distinct functional properties, although the molecular basis of this is unclear. Here we demonstrate IFN uniquely and specifically ligates to IFNAR1 in an IFNAR2-independent manner and provide the structural basis of the IFNAR1-IFN interaction. We show that the IFNAR1-IFN complex transduces signals to modulate the expression of a set of genes independently of IFNAR2. Moreover, we show the in vivo importance of the IFNAR1-IFN signaling axis in a murine model of LPS-induced septic shock. Thus, we provide a molecular basis for understanding specific functions of IFN?.
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| Overall design |
Interferon b induced gene expression in peritoneal exudate cells was measured 3hr post intra-peritoneal injection of 10,000IU/ml of interferon beta or saline into wildtype and Ifnar2-/- mice. Three independant experiments were performed for each treatment in both genotypes using different mice for each sample.
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| Contributor(s) |
Hertzog P, De Weerd N, Gould J, Forster S |
| Citation(s) |
23872679 |
| Submission date |
Jul 12, 2013 |
| Last update date |
May 10, 2018 |
| Contact name |
Sam Forster |
| Organization name |
Monash University
|
| Department |
Monash Institute
|
| Lab |
Centre for Innate Immunity and Infectious Diseases
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| Street address |
27 - 31 Wright Street
|
| City |
Clayton |
| State/province |
Victoria |
| ZIP/Postal code |
3168 |
| Country |
Australia |
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| Platforms (1) |
| GPL4134 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Feature Number version) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA211861 |
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