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| Status |
Public on Dec 31, 2013 |
| Title |
Expression data from rho+ and rho0 budding yeast celles harvetsed from complete medium with 2% glucose before or after shifting to complete medium without glucose |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by array
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| Summary |
This experiment compares mRNA expression in cells with and without mitochondrial DNA (ρ+ and ρ0 cells respectively). We used global RNA profiling to measure mRNA expression in ρ+ and ρ0 cells harvested at log phase from synthetic medium with glucose (CM + 2% glucose, "CMD"), and at 4 hours after transfer to the same medium without glucose (CM).
This profiling provided new clues about the ways in which nutirent signaling is reprogrammed in cells lacking mitocondrial DNA.
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| Overall design |
mRNA expression was assessed by analyzing the RNA from three biological replicates obtained under four conditions: ρ+ cells in glucose, ρ+ cells no glucose, ρ0 cells in glucose, ρ0 cells no glucose.
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| Contributor(s) |
Friis RM, Schultz MC |
| Citation missing |
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| Submission date |
Jul 18, 2013 |
| Last update date |
Jul 01, 2016 |
| Contact name |
Michael C. Schultz |
| E-mail(s) |
michael.schultz@ualberta.ca
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| Organization name |
University of Alberta
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| Department |
Biochemistry
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| Street address |
MSB 4-73
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| City |
Edmonton |
| State/province |
Alberta |
| ZIP/Postal code |
T6G 2H7 |
| Country |
Canada |
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| Platforms (1) |
| GPL90 |
[YG_S98] Affymetrix Yeast Genome S98 Array |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA212554 |
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