|
| Status |
Public on Jun 09, 2014 |
| Title |
Genome wide profiling of RNA Polymerase II upon knock-down or overexpression of RECQL5 |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
RECQL5 globally effects the distribution of RNA Polymerase II on genes in a dose dependent manner. Knock-down of RECQL5 reduces RNA Polymerase II density in the gene body, while increasing density on TSS and TTS. Overexpression shows exactly the opposite effect. We postulate that RECQL5 acts as a regulator of the elongation rate, more specifically, that the knock-down increases elongation rate while the overexpression decreases it.
|
| |
|
| Overall design |
RNA Polymerase II ChIP-Seq upon knock-down of RECQL5 with either of two specific shRNAs or a CTRL shRNA, and upon Doxycycline induced overexpression of RECQL5 .
|
| |
|
| Contributor(s) |
Saponaro M, Svejstrup JQ |
| Citation(s) |
24836610 |
| Submission date |
Jul 18, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Richard James Mitter |
| Organization name |
The Francis Crick Institute
|
| Department |
Bioinformatics & Biostatistics
|
| Street address |
1 Midland Road
|
| City |
London |
| State/province |
LONDON |
| ZIP/Postal code |
NW1 1AT |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL10999 |
Illumina Genome Analyzer IIx (Homo sapiens) |
|
| Samples (9)
|
|
| This SubSeries is part of SuperSeries: |
| GSE49134 |
RECQL5 Controls Transcript Elongation and Suppresses Transcription-Associated Genome Instability |
|
| Relations |
| BioProject |
PRJNA212573 |
| SRA |
SRP027563 |
Less...
More...