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| Status |
Public on Jul 20, 2013 |
| Title |
Gene expression data from brain nucleus accumbens shell (Acb-sh) following binge-like alcohol drinking by adolescent alcohol-preferring rats |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
The objective of this study was to determine changes in gene expression within the extended amygdala following binge-like alcohol drinking by adolescent alcohol-preferring (P) rats. Starting at 28 days of age, P rats were given concurrent access to 15 and 30 % ethanol for 3 one-h sessions for 5 consecutive days each week until they were 49 days old. Rats were killed by decapitation 3 h after the first ethanol access session on the 15th day of drinking. RNA was prepared from micropunch samples of the nucleus accumbens shell (Acb-sh) and central nucleus of the amygdala (CeA). Ethanol intakes were 2.5 – 3.0 g/kg/session. There were 154 and 182 unique named genes that significantly differed (FDR = 0.2) between the water and ethanol group in the Acb-sh and CeA, respectively. Gene Ontology (GO) analyses indicated that adolescent binge drinking produced changes in the in biological processes involved in cell proliferation and regulation of cellular structure in the Acb-sh, and in neuron projection and positive regulation of cellular organization in the CeA. Ingenuity Pathway Analysis indicated that, in the Acb-sh, there were several major intracellular signaling pathways (e.g., cAMP-mediated and protein kinase A signaling pathways) altered by adolescent drinking, with 3-fold more genes up-regulated than down-regulated in the alcohol group. The cAMP-mediated signaling system was also up-regulated in the CeA of the alcohol group. Weighted gene co-expression network analysis (WGCNA) indicated significant G-protein coupled receptor signaling and transmembrane receptor protein kinase signaling categories in the Acb-sh and CeA, respectively. Overall, the results of this study indicated that binge-like alcohol drinking by adolescent P rats is differentially altering the expression of genes in the Acb-sh and CeA, some of which are involved in intracellular signaling pathways and may produce long-term changes in neuronal function.
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| Overall design |
Differences in gene expression in brain nucleus accumbens shell (Acb-sh) were compared in two groups of alcohol-preferring (P) rats, one given water only and the other given access to 15 & 30% ethanol during adolescence.
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| Contributor(s) |
McBride WJ, Kimpel MW, McClintick JN, Ding Z, Edenberg HJ, Liang T, Rodd ZA, Bell RL |
| Citation(s) |
24355552 |
| Submission date |
Jul 19, 2013 |
| Last update date |
Jul 31, 2017 |
| Contact name |
Mark William Kimpel |
| E-mail(s) |
mwkimpel@gmail.com
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| Organization name |
Indiana University
|
| Department |
Psychiatry
|
| Lab |
McBride
|
| Street address |
15032 Hunter Court
|
| City |
Westfield |
| State/province |
IN |
| ZIP/Postal code |
46074 |
| Country |
USA |
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| Platforms (1) |
| GPL1355 |
[Rat230_2] Affymetrix Rat Genome 230 2.0 Array |
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| Samples (20)
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| This SubSeries is part of SuperSeries: |
| GSE49042 |
Changes in Gene Expression within the Extended Amygdala Following Binge-Like Alcohol Drinking by Adolescent Alcohol-Preferring (P) Rats |
|
| Relations |
| BioProject |
PRJNA212739 |
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