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| Status |
Public on Jul 23, 2013 |
| Title |
Transcriptome analysis of the D3 mouse embryonic stem cell line in comparison to the MIN6 pancreatic beta-cell insulinoma cell line |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Analysis of the transcriptional differences between mouse ES cells from the D3 cell line and transformed beta-cells (MIN6 cell line) as determined by a direct comparative analysis of their transcriptome. The results show that 40% of transcripts were differentially expressed between D3 and MIN6 cells. There is thus a marked difference in the pattern of transcription between the ES cell and beta-cell genomes.
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| Overall design |
Total RNA was extracted from three independent replicates of passage 21 D3 cells and passage 23 MIN6 cells. A whole mouse genome comparison between D3 and MIN6 cells was performed using the Mouse WG-6 v2.0 Expression Beadchip from Illumina.
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| Contributor(s) |
Wong JY, Jack MM, O'Neill C |
| Citation(s) |
24845830 |
| Submission date |
Jul 22, 2013 |
| Last update date |
Oct 23, 2019 |
| Contact name |
Chris O'Neill |
| E-mail(s) |
chris.oneill@sydney.edu.au
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| Organization name |
University of Sydney
|
| Department |
Kolling Institute of Medical Research
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| Lab |
Centre for Developmental and Regenerative Medicine
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| Street address |
Kolling Institute of Medical Research, Royal North Shore Hospital, Reserve Road, St Leonards
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| City |
Sydney |
| ZIP/Postal code |
2065 |
| Country |
Australia |
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| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA212924 |
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