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| Status |
Public on Mar 14, 2015 |
| Title |
RNA sequencing analysis of abdominal adipose tissue in 7 week old juvenile broiler chickens divergently selected for high or low body weight |
| Organism |
Gallus gallus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Chickens divergently selected for either high growth (HG genotype) or low growth (LG genotype) at SRA-INRA, France were used to profile abdominal adipose gene expression at 7 wk of age. The HG and LG chickens are different in various phenotypic and metabolic measurements, including growth rate, abdominal fat, plasma glycemia, insulinemia, T4, T3, triglyceride and NEFA. The HG and LG chickens are valuable as a model for biomedical and agricultural traits. Massively parallel RNA sequencing (RNA-Seq) was completed on an Illumina HiSeq 2000 System for transcription analysis of HG and LG abdominal fat. Need information on data processing, statistical analysis, and differential expression.
Keywords: abdominal fat, divergently selected chickens, growth, transcriptional profiling, differentially expressed genes
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| Overall design |
Abdominal fat mRNA profiles of high growth (HG genotype) or low growth (LG genotype) chickens at 7 weeks of age were generated by deep sequencing (on an Illumina HiSeq 2000 system).
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| Contributor(s) |
Resnyk CW, Le Bihan-Duval E, Duclos MJ, Simon J, Cogburn LA, Porter TE, Aggrey SE, Abasht B |
| Citation(s) |
28814270 |
| Submission date |
Jul 23, 2013 |
| Last update date |
Aug 02, 2019 |
| Contact name |
Larry Albert Cogburn |
| E-mail(s) |
cogburn@udel.edu
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| Phone |
302-831-1335
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| Organization name |
University of Delaware
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| Department |
Animal and Food Sciences
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| Lab |
Avian Functional Genomics
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| Street address |
531 South College Ave.
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| City |
Newark |
| State/province |
DE |
| ZIP/Postal code |
19717 |
| Country |
USA |
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| Platforms (1) |
| GPL16133 |
Illumina HiSeq 2000 (Gallus gallus) |
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| Samples (16)
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| GSM1194012 |
Chicken Abdominal fat, high growth (HG), 7 week of age, replicate 1, technical replicate 1 |
| GSM1194013 |
Chicken Abdominal fat, high growth (HG), 7 week of age, replicate 2, technical replicate 1 |
| GSM1194014 |
Chicken Abdominal fat, high growth (HG), 7 week of age, replicate 3, technical replicate 1 |
| GSM1194015 |
Chicken Abdominal fat, high growth (HG), 7 week of age, replicate 4, technical replicate 1 |
| GSM1194016 |
Chicken Abdominal fat, low growth (LG), 7 week of age, replicate 1, technical replicate 1 |
| GSM1194017 |
Chicken Abdominal fat, low growth (LG), 7 week of age, replicate 2, technical replicate 1 |
| GSM1194018 |
Chicken Abdominal fat, low growth (LG), 7 week of age, replicate 3, technical replicate 1 |
| GSM1194019 |
Chicken Abdominal fat, low growth (LG), 7 week of age, replicate 4, technical replicate 1 |
| GSM1194020 |
Chicken Abdominal fat, high growth (HG), 7 week of age, replicate 1, technical replicate 2 |
| GSM1194021 |
Chicken Abdominal fat, high growth (HG), 7 week of age, replicate 2, technical replicate 2 |
| GSM1194022 |
Chicken Abdominal fat, high growth (HG), 7 week of age, replicate 3, technical replicate 2 |
| GSM1194023 |
Chicken Abdominal fat, high growth (HG), 7 week of age, replicate 4, technical replicate 2 |
| GSM1194024 |
Chicken Abdominal fat, low growth (LG), 7 week of age, replicate 1, technical replicate 2 |
| GSM1194025 |
Chicken Abdominal fat, low growth (LG), 7 week of age, replicate 2, technical replicate 2 |
| GSM1194026 |
Chicken Abdominal fat, low growth (LG), 7 week of age, replicate 3, technical replicate 2 |
| GSM1194027 |
Chicken Abdominal fat, low growth (LG), 7 week of age, replicate 4, technical replicate 2 |
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| Relations |
| BioProject |
PRJNA213101 |
| SRA |
SRP028166 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE49121_gene_exp.diff.gz |
603.7 Kb |
(ftp)(http) |
DIFF |
| GSE49121_genes.count_tracking.txt.gz |
372.3 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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