|
| Status |
Public on Jun 09, 2014 |
| Title |
Mapping of nascent RNA upon release of DRB in WT and KD of RECQL5 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Global Run-On has been performed on WT or KD for RECQL5 cells after release from DRB. When RECQL5 is knocked-down the transcriptional wave front is more advanced, suggesting that transcription is faster.
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| |
|
| Overall design |
Constitutive knock-down cell lines expressing or not endogenous levels of shRNA resistant RECQL5 under a Doxycycline inducible promoter were treated with high doses of DRB to block transcription. Upon release into fresh medium we were able to follow how much and how fast the RNA Pol II progresses through genes by mapping nascent RNA by Run-On. The experiment was performed in two cell line clones.
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| |
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| Contributor(s) |
Saponaro M, Svejstrup JQ |
| Citation(s) |
24836610 |
| Submission date |
Jul 23, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Richard James Mitter |
| Organization name |
The Francis Crick Institute
|
| Department |
Bioinformatics & Biostatistics
|
| Street address |
1 Midland Road
|
| City |
London |
| State/province |
LONDON |
| ZIP/Postal code |
NW1 1AT |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL10999 |
Illumina Genome Analyzer IIx (Homo sapiens) |
|
| Samples (16)
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| This SubSeries is part of SuperSeries: |
| GSE49134 |
RECQL5 Controls Transcript Elongation and Suppresses Transcription-Associated Genome Instability |
|
| Relations |
| BioProject |
PRJNA213130 |
| SRA |
SRP028170 |
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