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Series GSE4914 Query DataSets for GSE4914
Status Public on Oct 17, 2006
Title RNAi analysis of the FBP transactivator family
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The FBP (FUSE Binding Protein) transcription factor family is composed of three highly related members; FBP/FBP1, FBP2, and FBP3. While they all share distinct modular protein structures and nucleic acid-binding specificities, their transactivator strength is graded and they also differ in their interaction with their protein partners. Especially from the extensive nuclear co-localization of these molecules, we anticipated that they co-regulate a set of common target genes. Thus we knocked down the level of each FBP using specific siRNA and obtained an initial assessment of the degree to which these factors act concertedly or independently.
Keywords: binding site identification design
 
Overall design Each FBP family member was knocked down by transiently transfecting siRNAs, and the total RNA was compared after two days against the control RNA from the negative control transfection. Six arrays were hybridized and analyzed for human primary skin fibroblast samples; two independent experimental sets, each for the three different FBPs.
 
Citation(s) 16914741
Submission date May 24, 2006
Last update date Mar 16, 2012
Contact name David Levens
E-mail(s) levens@helix.nih.gov
Phone 301-496-2176
Fax 301-402-0043
Organization name NIH
Department NCI/CCR
Lab LP
Street address 10 Center Drive
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platforms (1)
GPL3779 NCI/ATC Hs-OperonV3
Samples (6)
GSM110526 siFBP1_Set A - 1st array - mAdbID:73261
GSM110527 siFBP3_Set A - 1st array - mAdbID:73262
GSM110528 siFBP2_Set A - 1st array - mAdbID:73427
Relations
BioProject PRJNA95919

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE4914_RAW.tar 23.6 Mb (http)(custom) TAR (of GPR)
Raw data provided as supplementary file

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