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Series GSE49254 Query DataSets for GSE49254
Status Public on Apr 23, 2015
Title Impact of conditional autophagy knockout on cytotoxic CD8 T cells at day 7 and day 8 after influenza infection
Organism Mus musculus
Experiment type Expression profiling by array
Summary To investigate the role of autophagy on cytotoxic CD8 T cells, we developed a new mouse that expresses an Atg5 gene with loxP sites up- and downstream of the exon 3, Cre-recombinase, T cell receptor specific for the OVA peptide SIINFEKL and the congenic marker Thy1.1. These cells were adoptively transferred into wild type mice followed by influenza-OVA infection and induction of Atg5 knockout at day 5 after infection. Tracking the kinetic of the cells show, that the Atg5 knockout cells do not expand as much as the control cells and they experience complete contraction within few days. No memory population is established. To investigate differences in Atg5 knockout cells versus control cells we sorted the donor cells at the peak of immune response and investigated their gene expression profile via microarray analysis.
 
Overall design 10.000 naive CD8 T cells were isolated from the spleens of transgenic mice (untreated) and were adoptively transferred into naive wild type mice. On the next day these mice got intranasally infected with 15 plaque forming units of Influenza strain A/PR8-OVA. At day 5 to 8, Atg5 knockout was induced by intraperitoneal tamoxifen administration. At day 7 and 8, donor cells were sorted from spleens, RNA was isolated and investigated via microarray. Three groups of transgenic cells were compared in two independent experiments: Atg5 knockout cells were compared to two groups of control cells: 1. heterozygously Atg5 loxP expressing cells and 2. homozygously Atg5 loxP expressing cell that lacked the expression of Cre-recombinase.
 
Contributor(s) Schlie K, Lum JJ
Citation(s) 25833396
Submission date Jul 26, 2013
Last update date Feb 02, 2018
Contact name Julian J. Lum
E-mail(s) jjlum@bccancer.bc.ca
Organization name BC Cancer Agency
Department Deeley Research Centre
Street address 2410 Lee Avenue
City Victoria
State/province BC
ZIP/Postal code V8R 6V5
Country Canada
 
Platforms (1)
GPL10787 Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Probe Name version)
Samples (12)
GSM1196001 control 2, day 7, repeat 1
GSM1196002 Atg5 KO, day 7, repeat 1
GSM1196003 control 1, day 7, repeat 1
Relations
BioProject PRJNA213416

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE49254_RAW.tar 38.0 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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