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Series GSE4927 Query DataSets for GSE4927
Status Public on Apr 30, 2007
Title Effect of OMP on gene expression in the olfactory epithelium
Organism Mus musculus
Experiment type Expression profiling by array
Summary OMP is expressed only in mature olfactory sensory neurons, arguing that its function is specially suited to the needs of OSNs. However none of the properties of OMP suggest any direct role in regulating gene expression in OSNs. Our data confirms that gene expression in the olfactory epithelium of mice lacking OMP is indistinguishable from mice expressing OMP. We used affymerix M430v2.0 gene chips to cover as much of genome as possible and observed no statistically significant differences in mRNA abundance between the two genotypes.These results imply that OMPs ability to regulate signal transduction with in OSNs has little effect on gene expression and that its ability to promote mitosis in neighbouring cells (under culture conditions) is insufficiently active under normal laboratory housing conditions to generate detectable differences in gene expression patterns.
Keywords: genetic modification
Overall design The overall design of the experiment is to undertand the effect of OMP (olfactory marker protein) on gene expression in the olfactory epithelium. We isolated the olfactory epithelium from homozygous and heterozygous OMP-GFP mice, extracted the RNA and hybridized on to the mouse expression set 430 2.0 Chip.
Contributor(s) Sammeta N, Yu T, McClintock TS
Citation(s) 17444493
Submission date May 25, 2006
Last update date Feb 11, 2019
Contact name Neeraja Sammeta
Organization name University of Kentucky
Department Physiology
Lab McClintock
Street address 800 Rose Street,
City Lexington
State/province KY
ZIP/Postal code 40536
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (6)
GSM111137 OMP-GFP homozygous 1
GSM111138 OMP-GFP homozygous 2
GSM111139 OMP-GFP homozygous 3
BioProject PRJNA95873

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