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Series GSE4936 Query DataSets for GSE4936
Status Public on Apr 20, 2007
Title Transcriptional Profiling of Hh-Treated Embryoid Bodies
Organism Mus musculus
Experiment type Expression profiling by array
Summary This experiment was specifically designed to measure neural targets of Shh signaling, we sought to profile the genes upregulated by Hh signaling in the ventral neural tube to obtain a valid dataset. To obtain ventral-specific markers, we generated retinoic acid-treated EBs grown in the presence or absence of HH-Ag. We did not observe induction of ventral Hh markers in RA-treated constitutive Gli1FLAG EBs and used these for the control, baseline set. The presence of FoxA2, Nkx2.9 and Nkx6.1 amongst the top 10 genes based on expression levels suggests that profiling significantly enriches for Hh-dependent cell types. As expected, the benchmark standard Gli1 was not up-regulated in our array, since it is constitutively expressed in the control as well.
Keywords: neural progenitors, embryoid bodies, differentiation, Hedgehog, retinoic acid
Overall design There are a total of 8 samples. Four biological replicates of Retinoic-acid treated EBs (Baseline) and 4 additional biological samples of retinoic acid + Hh-Ag treated EBs (induced sample).
Contributor(s) Vokes SA, Ji H, Wong WH, McMahon AP
Citation(s) 17442700
Submission date May 26, 2006
Last update date Feb 11, 2019
Contact name Steve A. Vokes
Organization name University of Texas at Austin
Department Molecular Biosciences
Lab Steve Vokes
Street address 2500 Speedway
City Austin
State/province Texas
ZIP/Postal code 78757
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (8)
GSM111166 01_RA_EB
GSM111168 02_RA_EB
GSM111170 03_RA_EB
BioProject PRJNA95891

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE4936_RAW.tar 45.1 Mb (http)(custom) TAR (of CEL, EXP)
Raw data provided as supplementary file

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