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Series GSE49556 Query DataSets for GSE49556
Status Public on Sep 15, 2013
Title The effect of miR-294 and miR-181 on the transcriptome during OSK reprogramming of MEFs
Organism Mus musculus
Experiment type Expression profiling by array
Summary Study designed to determine the immediate effects of supplementing OSK reprogramming with miR-294 or miR-181. MEFs were infected on day 0, and transfected with miR-294, miR-181 or control mimic on day 1. On day 3 RNA was extracted. OSK infected MEFs samples were compared to non-infected MEFs and to fully reprogrammed iPSCs.
 
Overall design Performed in biological triplicate, with each sample containing different OSK viral stocks, different preparations of MEFs and independent transfections. Biological triplicate iPSCs represent three independent and clonally expanded iPSC lines. Total RNA collected with Trizol. All processing conducted at the UCLA Neuroscience Genomics Core. MouseRef-8 v2.0 Expression BeadChips.
 
Contributor(s) Blelloch R, Judson RL
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Aug 05, 2013
Last update date Jun 14, 2018
Contact name Robert Blelloch
E-mail(s) robert.blelloch@ucsf.edu
Phone 415-476-2839
Organization name UCSF
Department Urology
Lab Blelloch
Street address RMB1018, 35 Medical Center Way
City San Francisco
State/province CA
ZIP/Postal code 94143
Country USA
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (15)
GSM1201707 miR294_OSK_Day3_Rep1
GSM1201708 iPSCs_Rep1
GSM1201709 miR181_OSK_Day3_Rep1
Relations
BioProject PRJNA214243

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE49556_RAW.tar 3.1 Mb (http)(custom) TAR
GSE49556_non-normalized.txt.gz 2.3 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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