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| Status |
Public on Jul 24, 2014 |
| Title |
In-culture titration experiment for sRSE |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
In order to test the transcriptome-wide functionality of the identified sRSE instances, we performed an in vivo titration experiment in which synthetic RNA oligonucleotides harboring tandem sRSE1 repeats were used as intracellular decoys that would bind the putative trans factor, preventing it from targeting endogenous transcripts.
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| Overall design |
Capped and poly-adenylated RNAs carrying tandem sRSE elements were transfected along with scrambled RNA molecules as control. 48 hours post-transfection, samples were subjected to transcriptome profiling to measure the regulatory consequences of the in-vivo titration of the sRSE-binding trans factor.
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| Contributor(s) |
Goodarzi H, Zhang S, Buss CG, Fish L, Tavazoie S, Tavazoie SF |
| Citation(s) |
25043050 |
| Submission date |
Aug 07, 2013 |
| Last update date |
Aug 13, 2018 |
| Contact name |
Hani Goodarzi |
| Organization name |
UCSF
|
| Department |
Biochemistry and Biophysics
|
| Street address |
600 16th St, GH S312D
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94158 |
| Country |
USA |
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| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE49649 |
Metastasis-suppressor transcript destabilization through TARBP2 binding of mRNA hairpins |
|
| Relations |
| BioProject |
PRJNA214607 |
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