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Series GSE4963 Query DataSets for GSE4963
Status Public on Jul 21, 2006
Title Heart Failure, DITPA and Captopril
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary Three weeks after infarction, age matched rats with large infarction by ECG criteria were randomly assigned to one of the three groups: 1) Treatment with Captopril for 21 days and a daily subcutaneous dose of DITPA for the last 10 days of treatment, 2) Treatment for 10 days with DITPA alone, 3) Control heart failure and 4) Sham-operated.
Keywords: Drug Effects on Heart Failure
Overall design Total RNA was prepared from left ventricle using TRIzol and later Poly A+ RNA was isolated with Oligotex beads. About 0.5ug Poly A+ RNA was reverse transcribed and labeled with Alexa Fluor 546 and 647. Labeled probed were hybridized with Operon's rat Unigene oligo library printed on glass slides. After 2 lows and 1 high stringency washes, Prolong Antifade was added to the slides to prevent any bleaching effect. The experiments were repeated 2-4 times by dye-swaping. Slides were scanned in Array Worx scanner with dual wave length. The data ratio was normalized using a location and intensity dependent Lowess formula.
Contributor(s) Maitra N, Greer K, Morkin E
Citation(s) 18030062
Submission date Jun 01, 2006
Last update date Mar 16, 2012
Contact name Niranjan Maitra
Phone 520-626-4966
Fax 520-626-8408
Organization name University of Arizona
Department Sarver Heart Center
Lab Eugene Morkin
Street address 1501 North Campbell Avenue
City Tucson
State/province AZ
ZIP/Postal code 85724
Country USA
Platforms (1)
GPL3801 Operon Rat Oligonucleotide Array
Samples (20)
GSM111772 Effect of drugs, DITPA and Captopril on rat heart failure-File 49
GSM111823 Effect of drugs, DITPA and Captopril on rat heart failure-File 50
GSM111824 Effect of drugs, DITPA and Captopril on rat heart failure-File 51
BioProject PRJNA96719

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