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| Status |
Public on Aug 09, 2013 |
| Title |
Deep sequencing of endogenous mRNA from Caenorhabditis elegans in the presence and absence of 1-methylnicotinamide (MNA) and nicotinic acid (NA) |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Sirtuins, a family of histone deacetylases, have a fiercely debated role in regulating lifespan of different species.
Contrasting recent observations, we here find that overexpression of sir-2.1, the orthologue of mammalian SirT1, does extend C. elegans lifespan.
Sirtuins are known to convert NAD+ into nicotinamide (NAM).
We here find that NAM and its metabolite, 1-methylnicotinamide (MNA), extend C. elegans lifespan, also in the absence of sir-2.1.
Consistently, impairment of sir-2.1 prevents extension of lifespan by nicotinic acid (NA), a NAD+ precursor.
Taken together, sirtuins extend lifespan by promoting formation of MNA to generate a phase I - mediated ROS signal, providing an unexpected mechanistic role for sirtuins beyond histone deacetylation.
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| Overall design |
9 samples: 3 mRNA profiles of C.elegans 48h after L4 exposed to nicotinic acid; 3 mRNA profiles of C.elegans 48h after L4 exposed to 1-MNA; 3 mRNA profiles of C.elegans 48h after L4 as controls (H20)
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| Contributor(s) |
Schmeisser K, Mansfeld J, Kuhlow D, Weimer S, Priebe S, Heiland I, Birringer M, Groth M, Segref A, Kanfi Y, Price NL, Schmeisser S, Schuster S, Pfeiffer A, Guthke R, Platzer M, Hoppe T, Cohen HY, Zarse K, Sinclair DA, Ristow M |
| Citation(s) |
24077178 |
| Submission date |
Aug 08, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Marco Groth |
| E-mail(s) |
dnaseq@leibniz-fli.de
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| Organization name |
Leibniz Institute on Aging - FLI
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| Department |
Core Facility - Next Generation Sequencing
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| Street address |
Beutenbergstraße 11
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| City |
Jena |
| ZIP/Postal code |
07747 |
| Country |
Germany |
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| Platforms (1) |
| GPL13657 |
Illumina HiSeq 2000 (Caenorhabditis elegans) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA214676 |
| SRA |
SRP028602 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE49662_ce_Nr101-109_counts.txt.gz |
551.0 Kb |
(ftp)(http) |
TXT |
| GSE49662_ce_Nr101-109_rpkm.txt.gz |
1.2 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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