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Series GSE4974 Query DataSets for GSE4974
Status Public on Aug 25, 2006
Title thrombopoietin-induced megakaryocyte differentiation with and without nicotinamide
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Culture of hematopoietic stem and progenitor cells in the presence of thrombopoietin induces megakaryocytic differentiation. Addition of nicotinamide to thrombopoietin-stimulated cultures results in significant increases in megkaryocytic differentiation including greater polyploidization and enhanced proplatelet formation. This study focuses on understanding the differences in the temporal gene expression pattern during differentiation with and without nicotinamide. Nicotinamide was added on day 5.
Keywords: treatment response
Overall design Two biological experiments were analyzed. For each experiment, a sample was taken on day 5, before nicotinamide addition, and on days 6, 8, and 10 from both nicotinamide and control treated cultures. Each sample was analyzed in duplicate for a total of 14 hybridizations per culture or 28 total hybridizations.
Contributor(s) Fuhrken PG, Giammona LM, Papoutsakis ET, Miller WM
Citation(s) 17054670
Submission date Jun 02, 2006
Last update date Dec 06, 2012
Contact name Eleftherios Terry Papoutsakis
Organization name University of Delaware
Department Chemical Engineering
Street address 15 Innovation Way
City Newark
State/province DE
ZIP/Postal code 19711
Country USA
Platforms (1)
GPL887 Agilent-012097 Human 1A Microarray (V2) G4110B (Feature Number version)
Samples (28)
GSM112271 Donor MK09 Pre-treat Day 5 rep1
GSM112272 Donor MK09 Pre-treat Day 5 rep2
GSM112273 Donor MK09 thrombopoietin Day 06 rep1
BioProject PRJNA95477

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Supplementary file Size Download File type/resource
GSE4974_RAW.tar 3.3 Mb (http)(custom) TAR

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