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Status |
Public on Aug 14, 2013 |
Title |
Misregulation of the broad-range phospholipase C activity increases the susceptibility of Listeria monocytogenes to intracellular killing by neutrophils |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
Listeria monocytogenes is a facultative intracellular bacterial pathogen that tightly regulates the activities of various virulence factors during infection. A mutant strain (the plcBΔpro mutant) that has lost the ability to control the activity of a phospholipase C (PC-PLC) is attenuated a hundred fold in mice. This attenuation is not due to a lack of bacterial fitness, but appears to result from a modified host response to infection. The transcriptomic pattern of immunerelated genes in infected macrophages indicated no differential response to wild-type L. monocytogenes vs the plcBΔpro mutant.
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Overall design |
Cultures of bone marrow derived macrophages from BALB/c were infected with either wild type or mutant L. monocytogens for 3, 6, or 9 hrs. The macrophages were then collected and RNA isolated for microarray analysis of gene expression.
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Contributor(s) |
Blank BS, AbiAbdallah DS, Park JJ, Nazarova EV, PavinskiBitar A, Maurer KJ, Marquis H |
Citation missing |
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Submission date |
Aug 13, 2013 |
Last update date |
Jan 12, 2017 |
Contact name |
Helene Marquis |
E-mail(s) |
hm72@cornell.edu
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Organization name |
Cornell University
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Department |
Microbiology & Immunology
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Street address |
Vet Medical Center, Room C5-169
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City |
Ithaca |
State/province |
NY |
ZIP/Postal code |
14853 |
Country |
USA |
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Platforms (1) |
GPL7202 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version) |
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Samples (24)
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Relations |
BioProject |
PRJNA215020 |