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| Status |
Public on Aug 15, 2016 |
| Title |
Chromatin organization in NPP-13 knockdown C. elegans embryos [seq] |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
C. elegans nuclear pore protein NPP-13 associates with small RNA genes transcribed by RNA Polymerase III. To test if the nuclear pore-chromatin interactions play a role in large-scale chromatin organization, we determined nuclear membrane-genome interactions and RNA Polymerase II localization in C. elegans embryos depleted for NPP-13.
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| Overall design |
Genome-wide ChIP-seq and ChIP-chip for nuclear membrane protein LEM-2, RNA Polymerase II (AMA-1) and H3K4me3 were performed in mixed-stage C. elegans embryos depleted for NPP-13. As a control, ChIP was also performed in wild-type embryos treated with empty vector.
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| Citation missing |
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| Submission date |
Aug 15, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Kohta Ikegami |
| E-mail(s) |
kohta.ikegami@cchmc.org
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| Organization name |
Cincinnati Children's Hospital Medical Center
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| Department |
Division of Molecular Cardiovascular Biology
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| Lab |
Ikegami Lab
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| Street address |
240 Albert Sabin Way, Cincinnati, OH 45229
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| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45229 |
| Country |
USA |
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| Platforms (1) |
| GPL13657 |
Illumina HiSeq 2000 (Caenorhabditis elegans) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE50488 |
Chromatin organization in NPP-13 knockdown C. elegans embryos |
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| Relations |
| BioProject |
PRJNA217750 |
| SRA |
SRP029356 |
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