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Series GSE49940 Query DataSets for GSE49940
Status Public on Dec 10, 2014
Title Genome-wide analysis of gene expression during somatic cell reprogramming
Organism Mus musculus
Experiment type Expression profiling by array
Summary Reprogrammed mouse embryonic fibroblasts generated clonal cell lines were analysed during maintained expression of the four reprogramming transcription factors (C-Myc, Oct4, Sox2, Klf4). The transgene expressing clonal cell lines segregated in to two Nanog positive cell types, F-class and C-class, that both differentiate in to three germ layers. The emergence of F-class cells is dependent on the expression of all four factors with a necessity for high levels of transgene expression. The F-class cells represent a novel and stable cell state.
Overall design Mouse embryonic fibroblasts were reprogrammed using four transcription factors (C-Myc, Oct4, Sox2, Klf4) and clonal cell lines established. Cell lines were maintained in doxycycline supplemented media to sustain transgene expression. Global gene expression profile analysis was performed on the clonal cell lines at day16, with respect to embryonic stem cells and parental fibroblast cells. F-class cells (clone 1) were maintained for a further two weeks and found not to acquire expression of ESC associated genes.
Contributor(s) Nagy A, Tonge PD
Citation(s) 25503232
Submission date Aug 16, 2013
Last update date Jun 14, 2018
Contact name Andras Nagy
Organization name Lunenfeld Tanenbaum Research Institute
Department Mount Sinai Hospital
Street address 25 Orde street
City Toronto
ZIP/Postal code M5T3H7
Country Canada
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (51)
GSM1544133 Primary MEF
GSM1544134 Pri clone 1
GSM1544135 Pri clone 2
BioProject PRJNA215403

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE49940_RAW.tar 3.1 Mb (http)(custom) TAR
GSE49940_non-normalized.txt.gz 6.3 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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