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Series GSE49954 Query DataSets for GSE49954
Status Public on Aug 19, 2013
Title T lymphocytes from Chronic HCV-infected patients express unique pro-apoptotic gene signature.
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Although extensive studies have demonstrated the gene expression patterns of antigen-specific CD4+ and CD8+ T-cells during chronic hepatitis C virus (HCV) infection, the transcriptional profiles of global CD4+ and CD8+ T-cells remains unclear. In this report, we recruited 10 long-term (~20 years) treatment-naïve chronic HCV (CHC) patients and 5 healthy donors (HDs) to investigate differences in global CD4+ and CD8+ T-cells gene expression profile.
Global CD4+ and CD8+ T-cells showed unique transcriptional profiles in the expression of apoptosis-related genes. We identified BCL2, PMAIP1, and CASP1 in CD4+ T-cells and IER3 and BCL2A1 in CD8+ T-cells from CHC patients as HCV-specific gene signatures. The unique apoptosis-related gene expression profilesin global CD4+ and CD8+ T-cells programmed by chronic HCV infection seemed to enhance activation-induced apoptosis, which was suffered by global CD4+ and CD8+ T-cells.
Overall design We obtained 15 blood samples to identify the gene expression signatures of global CD4+ and CD8+ T-cells due to chronic HCV infection. The samples included: 5 samples from high HCV viral load patients (HCV-h), 5 samples from low HCV viral load (HCV-l) and 5 samples from healthy donors (HD). HCV patients were all Ab+ and treatment-naive prior to the study. Samples were taken once from each individual. Global CD4+ and CD8+ T-cells were enriched by microbeads, and total RNA were used in gene chip analysis.
Contributor(s) Zhao B, Zheng S, Gong L, Wang Y, Chen C, Jin W, Zhang D, Yuan X, Guo J, Duan Z, He Y
Citation(s) 24130824
Submission date Aug 18, 2013
Last update date Nov 30, 2020
Contact name Binbin Zhao
Organization name Institute of Pathogen Biology, CAMS&PUMC
Street address No. 9 Dong Dan San Tiao, Dongcheng District
City Beijing
ZIP/Postal code 100730
Country China
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (30)
GSM1210739 HD A8-CD4+ T-cells
GSM1210740 HD A9-CD4+ T-cells
GSM1210741 HD A11-CD4+ T-cells
BioProject PRJNA215683

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Supplementary file Size Download File type/resource
GSE49954_RAW.tar 126.3 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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