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| Status |
Public on Aug 19, 2013 |
| Title |
T lymphocytes from Chronic HCV-infected patients express unique pro-apoptotic gene signature. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Although extensive studies have demonstrated the gene expression patterns of antigen-specific CD4+ and CD8+ T-cells during chronic hepatitis C virus (HCV) infection, the transcriptional profiles of global CD4+ and CD8+ T-cells remains unclear. In this report, we recruited 10 long-term (~20 years) treatment-naïve chronic HCV (CHC) patients and 5 healthy donors (HDs) to investigate differences in global CD4+ and CD8+ T-cells gene expression profile.
Global CD4+ and CD8+ T-cells showed unique transcriptional profiles in the expression of apoptosis-related genes. We identified BCL2, PMAIP1, and CASP1 in CD4+ T-cells and IER3 and BCL2A1 in CD8+ T-cells from CHC patients as HCV-specific gene signatures. The unique apoptosis-related gene expression profilesin global CD4+ and CD8+ T-cells programmed by chronic HCV infection seemed to enhance activation-induced apoptosis, which was suffered by global CD4+ and CD8+ T-cells.
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| Overall design |
We obtained 15 blood samples to identify the gene expression signatures of global CD4+ and CD8+ T-cells due to chronic HCV infection. The samples included: 5 samples from high HCV viral load patients (HCV-h), 5 samples from low HCV viral load (HCV-l) and 5 samples from healthy donors (HD). HCV patients were all Ab+ and treatment-naive prior to the study. Samples were taken once from each individual. Global CD4+ and CD8+ T-cells were enriched by microbeads, and total RNA were used in gene chip analysis.
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| Contributor(s) |
Zhao B, Zheng S, Gong L, Wang Y, Chen C, Jin W, Zhang D, Yuan X, Guo J, Duan Z, He Y |
| Citation(s) |
24130824 |
| Submission date |
Aug 18, 2013 |
| Last update date |
Nov 30, 2020 |
| Contact name |
Binbin Zhao |
| E-mail(s) |
zhbbzj81@gmail.com
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| Organization name |
Institute of Pathogen Biology, CAMS&PUMC
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| Street address |
No. 9 Dong Dan San Tiao, Dongcheng District
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| City |
Beijing |
| ZIP/Postal code |
100730 |
| Country |
China |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (30)
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| Relations |
| BioProject |
PRJNA215683 |
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