 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Aug 20, 2013 |
| Title |
Expression Data from 12 week old APCmin/+ and littermate matched Wildtype B6 mouse Terminal Ileum (TI) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
APCmin/+ mice develop spontaneous gastrointestinal polyposis due to a dominantly inhereited germline loss-of-function mutation in the tumor suppressor adenomatous polyposis coli (APC). Changes in intestinal immune activity have been documented to occur prior to the development of fulminate polyposis. Such changes are thought to contribute to disease development.
We used microarrays to describe the changing intestinal transcriptional landscape in APCmin/+ mice. Whole transcriptome profiling from polypotic and nonpolypotic intestinal sections of APC/min+ mice were examined in the early stages of disease, and compared to normal intestinal sections from littermate matched wildtype B6 mice.
|
| |
|
| Overall design |
Nonpolypotic (wildtype and APCmin/+) and Polypotic (APCmin/+) sections of terminal ileum were identified by visual inspection, and subsequently selected for RNA isolation and hydridzation to Affymetrix Mouse Genome 430A 2.0 Arrays. Interference from bacterial RNA was selected against using a probeset enriched in oligos extending into 3’-poly-A tails.
|
| |
|
| Contributor(s) |
Chung AY, Egilmez NK |
| Citation(s) |
25228656 |
| Submission date |
Aug 19, 2013 |
| Last update date |
May 04, 2018 |
| Contact name |
Allen Yicheng Chung |
| E-mail(s) |
allenchu@buffalo.edu
|
| Phone |
716-829-5783
|
| Organization name |
University at Buffalo
|
| Department |
Microbiology and Immunology
|
| Lab |
Egilmez
|
| Street address |
3435 Main Street
|
| City |
Buffalo |
| State/province |
NY |
| ZIP/Postal code |
14214 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL8321 |
[Mouse430A_2] Affymetrix Mouse Genome 430A 2.0 Array |
|
| Samples (10)
|
| GSM1210940 |
Nonpolyptic TI, Wildtype Littermate(12wks), biological rep1 |
| GSM1210941 |
Polypotic TI, APCmin/+ (12wks), biological rep1 |
| GSM1210942 |
Nonolypotic TI, APCmin/+ (12wks), biological rep1 |
| GSM1210943 |
Polypotic TI, APCmin/+ (12wks), biological rep2 |
| GSM1210944 |
Nonolypotic TI, APCmin/+ (12wks), biological rep2 |
| GSM1210945 |
Nonpolyptic TI, Wildtype Littermate(12wks), biological rep2 |
| GSM1210946 |
Polypotic TI, APCmin/+ (12wks), biological rep3 |
| GSM1210947 |
Nonolypotic TI, APCmin/+ (12wks), biological rep3 |
| GSM1210948 |
Polypotic TI, APCmin/+ (12wks), biological rep4 |
| GSM1210949 |
Nonolypotic TI, APCmin/+ (12wks), biological rep4 |
|
| Relations |
| BioProject |
PRJNA215724 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE49970_RAW.tar |
20.2 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...