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Series GSE49980 Query DataSets for GSE49980
Status Public on Oct 31, 2013
Title The transcriptomic response of rat hepatic stellate cells to endotoxin: implications for hepatic inflammation and immune regulation
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary Located in the perisinusoidal space of Disse, hepatic stellate cells (HSCs) communicate with all other liver cell types by physical association and / or by producing cytokines and chemokines. In liver disease and folllowing liver transplantation, elevated levels of endotoxin (bacterial lipopolysaccharide: LPS) stimulate HSCs to produce increased amounts of cytokines and chemokines. Transcriptomic analysis of cultured HSCs stimulated with LPS yields a survey of expression changes which potentially modulate the hepatic inflammatory and immune responses.
Overall design In two independent experiments, rat HSCs were cultured with or without 10 ng/ml LPS for 1h and 24h, then harvested for microarray analysis (i.e., 8 samples).
In the first of these experiments, cells also were also harvested after 3, 6 and 12 hours of LPS treatment, for a total of 11 samples.
Contributor(s) Harvey SA, Dangi A, Tandon A, Gandhi CR
Citation(s) 24349206
Submission date Aug 19, 2013
Last update date Jul 31, 2017
Contact name Stephen Alan Kenneth Harvey
Organization name University of Pittsburgh School of Medicine
Department Ophthalmology
Lab EEINS 1014
Street address 203 Lothrop St
City Pittsburgh
State/province PA
ZIP/Postal code 15213-2588
Country USA
Platforms (1)
GPL1355 [Rat230_2] Affymetrix Rat Genome 230 2.0 Array
Samples (11)
GSM1211051 Cultured HSCs-control-1h-rep1
GSM1211052 Cultured HSCs-LPS-1h-rep1
GSM1211053 Cultured HSCs-LPS-3h-rep1
BioProject PRJNA215744

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Supplementary file Size Download File type/resource
GSE49980.txt.gz 990.2 Kb (ftp)(http) TXT
GSE49980_RAW.tar 31.1 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data are available on Series record

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