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| Status |
Public on Aug 26, 2013 |
| Title |
Expression data from human Th1 and Th1Th17 cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We previously demonstrated that Th1Th17 cells are highly permissive to HIV-1, whereas Th1 cells are relatively resistant. Here, we investigated molecular mechanisms underlying these differences. Superior HIV replication in Th1Th17 vs. Th1 cells was regulated by entry and post-entry mechanisms.
We used microarrays to detail the gene expression signatures caracterizing Th1 cells from Th1Th17.
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| Overall design |
Primary human Th1 (CXCR3+CCR6- phenotype) and Th1Th17 (CXCR3+CCR6- phenotype) CD4+ T-cells were isolated by flow cytometry from HIV-uninfected, healthy donors. Cells were stimulated via CD3/CD28 for 3 days. The RNA was extracted and hybridized on the GeneChip® Human Genome U133 Plus 2.0 Array (Affymetrix).
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| Contributor(s) |
Bernier A, Cleret-Buhot A, Goulet J, Monteiro P, Gosselin A, DaFonseca S, Wacleche VS, Jenabian M, Routy J, Tremblay C, Ancuta P |
| Citation(s) |
24359430 |
| Submission date |
Aug 25, 2013 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Jean-Philippe Goulet |
| E-mail(s) |
jp.goulet@umontreal.ca
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| Organization name |
Université de Montréal
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| Street address |
C.P. 6128, succursale Centre-ville
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| City |
Montréal |
| State/province |
Québec |
| ZIP/Postal code |
H3X 3J7 |
| Country |
Canada |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA216967 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE50175_RAW.tar |
38.4 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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