|
| Status |
Public on Dec 16, 2013 |
| Title |
Identification of FUS RNA targets in HeLa cells |
| Organism |
Homo sapiens |
| Experiment type |
Other
|
| Summary |
We identified a landscape of FUS-binding RNA targets in HeLa cells. The majority of the FUS binding sites are in introns of pre-mRNAs and less are in exons and untranslated regions. Significant FUS binding in introns flanking cassette exons, long intron (>100kb) containing transcripts and noncoding RNAs were detected in our study. We specifically determined the function of FUS in regulating the alternative splicing of cassette exons. The top FUS-associated cassette exon is exon 7 of the pre-mRNA of FUS itself. We demonstrated that FUS is a repressor of its own exon 7 splicing. FUS autoregulates its own protein levels by exon 7 alternative splicing and nonsense mediated decay. Moreover, Amyotrophic Lateral Sclerosis (ALS) linked FUS mutants are deficient in FUS autoregulation.
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| |
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| Overall design |
CLIP-seq of FUS in HeLa cells
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| |
|
| Contributor(s) |
Zhou Y, Liu S, Hicks GG |
| Citation(s) |
24204307 |
| Submission date |
Aug 25, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Geoffrey Hicks |
| E-mail(s) |
hicksgg@cc.umanitoba.ca
|
| Organization name |
University of Manitoba, Manitoba Institue of Cell Biology
|
| Department |
Biochemsity & Medical Genetics
|
| Street address |
BMSB 669, 745 Bannatyne Avenue
|
| City |
Winnipeg |
| State/province |
Manitoba |
| ZIP/Postal code |
R3E 0J9 |
| Country |
Canada |
| |
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| Platforms (1) |
| GPL9115 |
Illumina Genome Analyzer II (Homo sapiens) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA216971 |
| SRA |
SRP029210 |
