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Series GSE50180 Query DataSets for GSE50180
Status Public on Jul 30, 2014
Title Gene expression profiles of NIH3T3 cells stably transfected with the plasmids encoding the wild type PreS/S gene or the nonsense mutant gene (sW182*) of HBV.
Organism Mus musculus
Experiment type Expression profiling by array
Summary Background and Aims: Whether hepatitis B virus (HBV) could play a direct role in hepatocarcinogenesis remains uncertain. The 3' end nonsense mutations of HBV PreS/S gene have been found to encode transcriptional transactivation activity, suggesting these mutations may contribute to HBV-associated oncogenesis. Recently, we have identified a potent oncogenic HBV surface (S) gene nonsense mutant sW182*. Results: Gene expression microarray study revealed that sW182* mutant was significantly enriched by gene sets associated with cell cycle regulation, DNA repair, or genome instability. The transforming growth factor-induced (TGFBI) gene was downregulated in the sW182* mutant clones, and irresponsive to TGF- treatment. The level of Cyclin D1, a negatively regulated TGFBI target, was highly elevated in sW182* mutant cells. Exogenous expression of TGFBI alleviated the oncogenic activity of sW182* in mouse xenograft study. In human HBV-related HCC cancerous tissue, expression of TGFBI was downregulated in 25 of the 55 (45%) patients. Conclusions: Dysregulation of transforming growth factor-induced (TGFBI) gene is involved in the oncogenic activity of the sW182* mutant of hepatitis B virus S gene. This has never been described before.
 
Overall design NIH3T3 cells were stably transfected with plasmids encoding the wild type PreS/S gene or the nonsense mutant (sW182*) of HBV PreS/S gene. Totoal RNAs were extracted from those stable clones for gene expression microarray analysis using Illumina MouseRef-8 V2 BeadChip.
 
Contributor(s) Jiang SS, Huang SF
Citation(s) 24662304
Submission date Aug 26, 2013
Last update date Jun 14, 2018
Contact name Shih Sheng Jiang
E-mail(s) ssjiang@nhri.org.tw
Organization name National Health Research Institutes
Department National Institute of Cancer Research
Street address No. 35 Keyan Road, Zhunan County
City Miaoli County
ZIP/Postal code 350
Country Taiwan
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (5)
GSM1215159 Vector (clone 4)
GSM1215160 WT (clone 3)
GSM1215161 WT (clone 8)
Relations
BioProject PRJNA216982

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE50180_RAW.tar 3.1 Mb (http)(custom) TAR
GSE50180_non-normalized.txt.gz 471.7 Kb (ftp)(http) TXT
Processed data included within Sample table
Raw data are available on Series record

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