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Series GSE5042 Query DataSets for GSE5042
Status Public on Jul 06, 2006
Title Complementary action of the PGC-1 coactivators in mitochondrial biogenesis and brown fat differentiation
Organism Mus musculus
Experiment type Expression profiling by array
Summary Mitochondria play an essential role in the ability of brown fat to generate heat, and the PGC-1 coactivators control several aspects of mitochondrial biogenesis. To investigate their specific roles in brown fat cells, we generated immortal preadipocyte lines from the brown adipose tissue of mice lacking PGC-1α. We could then efficiently knockdown PGC-1β expression by shRNA expression. Loss of PGC-1α did not alter brown fat differentiation but severely reduced the induction of thermogenic genes. Cells deficient in either PGC-1α or PGC-1β coactivators showed a small decrease in the differentiation-dependant program of mitochondrial biogenesis and respiration; however, this increase in mitochondrial number and function was totally abolished during brown fat differentiation when both PGC-1α and PGC-1β were deficient. These data show that PGC-1α is essential for brown fat thermogenesis but not brown fat differentiation, and the PGC-1 coactivators play an absolutely essential but complementary function in differentiation-induced mitochondrial biogenesis. Affymetrix microarray analysis of total RNA from wt, PGC-1α KO and PGC-1α KO
cells expressing an RNAi specific for PGC-1β knockdown was performed. Of the 461
mitochondrial genes analyzed, 181 were found to be at least 20% different between wt
and defective PGC-1α and β adipocytes (p < 0.05). More than 85% of these genes were downregulated in cells deficient for PGC-1alpha and PGC-1beta.
Keywords: Analysis of mitochondrial gene expression
Overall design Brown preadipocytes that were either WT, KO for PGC-1alpha, or KO for PGC-1alpha and deficient for PGC-1beta (knockdown through siRNA expression) were differentiated for seven days. RNA was made from biological replicates of the three different types of brown adipocytes (WT, KO expressing a control siRNA, KO expressing a siRNA specific for PGC-1beta knockdown).
Contributor(s) Uldry M, Yang W, St-Pierre J, Lin J, Seale P, Spiegelman BM
Citation(s) 16679291
Submission date Jun 09, 2006
Last update date Feb 11, 2019
Contact name Marc Uldry
Phone 6176324661
Organization name Dana Farber Cancer Institute
Street address 1 Jimmy Fund Way
City Boston
State/province MA
ZIP/Postal code 02139
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (6)
GSM113409 WT differentiated brown fat cells (rep1)
GSM113411 WT differentiated brown fat cells (rep2)
GSM113461 PGC-1alpha -/- differentiated brown fat cells expressing a control siRNA (rep1)
BioProject PRJNA96677

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