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Series GSE50444 Query DataSets for GSE50444
Status Public on Oct 01, 2013
Title Gene expression in organized and disorganized human breast epithelial cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary We have reported more than a dozen microenvironmental factors whose signaling must be integrated in order to effect an organized, functional tissue morphology. In order to identify underlying commonalities in gene transcription associated with the phenotype, we compared the gene expression of organized and disorganized epithelial cells of the HMT-3522 breast cancer progression series: the non-malignant S1 cells that form polarized spheres (‘acini’), the malignant T4-2 cells that form large tumor-like clusters, and the ‘phenotypically reverted’ T4-2 cells that polarize as a result of correction of the microenvironmental signaling.
 
Overall design 24 samples were analyzed: 5 samples were non-malignant S1 cells that formed organized spherical structures ('acini'), 5 samples were the isogenic malignant counterpart of S1 cells, namely the T4-2 cells, which grow into disorganized large cell clusters; and 14 samples of T4-2 cells that had been treated with different reverting agents, these reverting agents cause the T4-2 cells to 'revert' in phenotype so that they resemble the structures formed by S1 cells.
 
Contributor(s) Becker-Weimann S, Bissell MJ, Onodera Y, Rizki A
Citation(s) 27203208
Submission date Aug 29, 2013
Last update date Jul 15, 2019
Contact name Sabine Becker-Weimann
E-mail(s) SBecker-Weimann@lbl.gov
Organization name Lawrence Berkeley National Laboratory
Department Life Sciences
Lab Bissell
Street address 1 Cyclotron Rd
City Berkeley
State/province CA
ZIP/Postal code 94720
Country USA
 
Platforms (2)
GPL3921 [HT_HG-U133A] Affymetrix HT Human Genome U133A Array
GPL4685 [U133AAofAv2] Affymetrix GeneChip HT-HG_U133A Early Access Array
Samples (24)
GSM1219374 non-malignant S1 - sample 1
GSM1219375 non-malignant S1 - sample 2
GSM1219376 non-malignant S1 - sample 3
Relations
BioProject PRJNA217499

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE50444_RAW.tar 65.5 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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