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Series GSE50501 Query DataSets for GSE50501
Status Public on Sep 23, 2013
Title Ablation of coactivator Med1 switches the cell fate of dental epithelia to that generating hair [CL stage]
Organism Mus musculus
Experiment type Expression profiling by array
Summary Cell fate is defined by specific transcriptional program. Here, we provide evidence that the transcriptional coactivator, Mediator 1 (MED1), is critical in determining the cell fate of ectodermal epithelia. MED1 ablation disrupted enamel formation and generated hair adjacent to the incisors. Deletion of MED1 altered the differentiation of dental epithelia to one expressing epidermal and hair genes similar to the skin. The cellular switch from dental to epidermal/hair lineage was characterized by abnormalities in MED1 deficient dental epithelial stem cells residing in cervical loop. MED1 deficiency caused a failure of dental epithelial stem cells to commit to the dental stratum intermedium regulated by Notch signaling. Instead, MED1 deficient cells retained stem cell potentials expressing Sox2. These cells were eventually adopted an epidermal fate probably through calcium provided through capillary networks, which is originally utilized for enamel formation. Our results demonstrate that MED1 regulates Sox2/Notch1 regulated cell lineage determination in dental epithelia. Our study also shows a potential to regenerate hairs by using genetically engineered dental tissues or cells outside of the skin.
 
Overall design n=3 WT and KO (each sample contain dissected dental tissues from 3 mice combined)
 
Contributor(s) Hu L, Nguyen T, Fong FC, Bikle DD, Oda Y
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Submission date Aug 30, 2013
Last update date Jun 14, 2018
Contact name Yuko Oda
E-mail(s) yuko.oda@ucsf.edu
Phone 4152692370
Organization name VA Medical Center UCSF
Department Endocrine Research
Street address 111N-MB 360 1700 Owens St, Suite 360
City San Francisco
State/province CA
ZIP/Postal code 94158
Country USA
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (2)
GSM1220301 Control (WT) 10wk CL
GSM1220302 Knockout (KO) 10wk CL
This SubSeries is part of SuperSeries:
GSE50504 Ablation of coactivator Med1 switches the cell fate of dental epithelia to that generating hair
Relations
BioProject PRJNA217785

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE50501_RAW.tar 3.1 Mb (http)(custom) TAR
GSE50501_non_normalized.txt.gz 303.2 Kb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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