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| Status |
Public on Dec 23, 2014 |
| Title |
Experimentally identified targets of a subset of adenovirus 5-encoded miRNAs. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Human adenovirus 5 encodes a small set of miRNAs, which are generated by DICER-mediated processing of 2 larger precursors, the so-called virus-associated RNAs I and II. To identify targets of one of the major miRNA isoforms derived from virus-associated RNAI (mivaRNAI-137), we isolated Argonaute complexes of mivaRNAI-137-transfected cells and analyzed co-purifying RNAs by microarray analysis. RNAs enriched in Argonaute complexes of mivaRNAI-137-transfected cells compared to cells transfected with a control siRNA were identified and subjected to further validation. RNAs specifically associated with Argonaute-containining complexes of adenovirus 5-infected cells were identified as well.
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| Overall design |
9 sets of RNAs isolated from Argonaute-containing complexes of cells transfected with 2 types of adenovirus 5-encoded miRNAs or a negative control siRNA as well as the corresponding total RNA were subjected to microarray analysis.
Consequences on gene expression of hypoxic condition created by exposure of cells to 1% oxygen in a hypoxia chamber.
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| Contributor(s) |
Bellutti F, Maximilian K, Doris K, Reinhard K |
| Citation(s) |
25410853 |
| Submission date |
Sep 03, 2013 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Maximilian Kauer |
| E-mail(s) |
maximilian.kauer@ccri.at
|
| Organization name |
CCRI, St.Anna Children Cancer Research Institute
|
| Department |
Molecular Biology
|
| Lab |
Heinrich Kovar
|
| Street address |
Zimmermannplatz 10
|
| City |
Vienna |
| ZIP/Postal code |
1090 |
| Country |
Austria |
| |
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| Platforms (2) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
| GPL11532 |
[HuGene-1_1-st] Affymetrix Human Gene 1.1 ST Array [transcript (gene) version] |
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| Samples (28)
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| Relations |
| BioProject |
PRJNA217915 |
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