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Series GSE50621 Query DataSets for GSE50621
Status Public on Sep 05, 2013
Title Expression analyses of inducible c-Fos expressing kerationcytes
Organism Mus musculus
Experiment type Expression profiling by array
Summary Expression analyses comparing c-Fos expressing keratinocytes vs non-expressing controls.
Skin squamous cell carcinomas (SCCs) are the second most prevalent skin cancers. Chronic skin inflammation has been associated with the development of SCCs, but the contribution of skin inflammation to SCC development remains largely unknown. In this study we demonstrate that inducible expression of c-fos in the epidermis of adult mice is sufficient to promote inflammation-mediated epidermal hyperplasia leading to the development of pre-neoplastic lesions. Interestingly, c-Fos transcriptionally controls mmp10 and s100a7a15 expression in keratinocytes subsequently leading to CD4 T cell recruitment to the skin, thereby promoting epidermal hyperplasia. Combining inducible c-fos expression in the epidermis with a single dose of the carcinogen 7,12-Dimethylbenz(a)anthracene (DMBA) leads to the development of highly invasive SCCs, which are prevented by using the anti-inflammatory drug Sulindac. Moreover, human SCCs display a correlation between c-FOS expression and elevated levels of MMP10 and S100A15 proteins as well as CD4 T cell infiltration. Our studies demonstrate a bidirectional crosstalk between pre-malignant keratinocytes and infiltrating CD4 T cells in SCC development. Therefore, targeting inflammation along with the newly identified targets, such as MMP10 and S100A15, represent promising therapeutic strategies to treat SCCs.
 
Overall design 5 different time points measured in triplicate comparing Dox-treated vs untreated c-FostetON keratinocytes
 
Contributor(s) Briso E, Zapatka M, Wagner EF, Rogon ZM
Citation(s) 24029918
Submission date Sep 05, 2013
Last update date Jun 14, 2018
Contact name Marc Zapatka
Organization name German Cancer Research Center
Department B060 Molecular Genetics
Street address Im Neuenheimer Feld 580
City 69120
ZIP/Postal code Heidelberg
Country Germany
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (29)
GSM1224751 c-FostetON Keratinocyte. 6h. Replicate 1.
GSM1224752 c-FostetON Keratinocyte. 6h. Replicate 2.
GSM1224753 c-FostetON Keratinocyte. 6h DOX. Replicate 1.
Relations
BioProject PRJNA218096

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE50621_RAW.tar 3.1 Mb (http)(custom) TAR
GSE50621_non-normalized_data.txt.gz 6.4 Mb (ftp)(http) TXT
Processed data included within Sample table
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