|
| Status |
Public on Jan 01, 2015 |
| Title |
Gene expression profiles upon Meis1 knockout in Hoxa9/Meis1-induced AML |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Meis1 is found cooperatively activated with Hoxa7/a9 in AML, and it indeed promotes leukemogenic activities of Hoxa9. It is important to identify downstream target genes of Meis1 to understand its cooperative activity with Hoxa9 in leukemogenesis.
We used microarrays to detail the global programme of gene expression upon Meis1 knockout.
|
| |
|
| Overall design |
Murine primary bone marrow cells of the Rosa26-Cre-ERT2 knock-in mouse were transformed by retroviral transduction of Hoxa9 and floxed Meis1. The immortalized bone marrow cells were treated with 2 μM of 4-hydroxytamoxifen to delete Meis1 cDNA. Gene expression profiles were compared between the original Hoxa9/Meis1-expressing cells and Meis1 deleted (Hoxa9 only) cells.
|
| |
|
| Contributor(s) |
Yokoyama T, Nakamura T |
| Citation(s) |
27018596 |
| Submission date |
Sep 06, 2013 |
| Last update date |
Aug 06, 2018 |
| Contact name |
Takuro Nakamura |
| E-mail(s) |
takuro-ind@umin.net
|
| Phone |
81-3-3570-0462
|
| Organization name |
Japanese Foundation for Cancer Research
|
| Department |
The Cancer Institute
|
| Lab |
Carcinogenesis
|
| Street address |
3-8-31 Ariake, Koto-ku
|
| City |
Tokyo |
| ZIP/Postal code |
135-8550 |
| Country |
Japan |
| |
|
| Platforms (1) |
| GPL11180 |
[HT_MG-430_PM] Affymetrix HT MG-430 PM Array Plate |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA218195 |
Less...
More...