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Status |
Public on Sep 07, 2013 |
Title |
Gene expression profiles in rat lung following intratracheal instillation with single-wall carbon nanotubes |
Organism |
Rattus norvegicus |
Experiment type |
Expression profiling by array
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Summary |
Gene expression profiling of the rat lung following intratracheal instillation with single-wall carbon nanotubes (SWCNTs) was employed to gain insights into these molecular events. We attempted to characterize time-dependent changes in the gene expression until 754 days after intratracheal instillation with SWCNTs suspensions at 0.2 mg (L-SWCNT) and 0.4 mg (H-SWCNT) injected dose per rat, and to identify the shift from the acute-phase to the chronic-phase phase on the basis of evaluation at the molecular level.
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Overall design |
Groups of nine-week-old male Wistar rats (n= 6 per group/ time point) were intratracheally instilled with single-wall carbon nanotubes (SWCNTs) suspended in 0.4 ml distilled water including 0.1% Triton X-100 as a single injection 0.1 mg (L-SWCNT) and 0.4 mg (H-SWCNT) SWCNTs/ rat). Control groups received 0.1% Triton X-100 (vehicle control). After intratracheal instillation treatment, rats were housed within polycarbonate cages at a controlled temperature of 22 °C with a chow diet ad libitum, and dissected at 3 days, 7 days, 30 days, 90 days, 180 days, 365 days and 754 days post-instillation. Right lungs of anesthetized rats were perfused with physiological saline, excised, and used for DNA microarray analysis.
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Contributor(s) |
Fujita K, Fukuda M |
Citation(s) |
24911292 |
Submission date |
Sep 06, 2013 |
Last update date |
Sep 15, 2014 |
Contact name |
Katsuhide Fujita |
E-mail(s) |
ka-fujita@aist.go.jp
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Organization name |
National Institute of Advanced Industrial Science and Technology
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Department |
Research Institute of Science for Safety and Sustainability
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Street address |
Onogawa 16-1
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City |
Tsukuba |
ZIP/Postal code |
3058569 |
Country |
Japan |
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Platforms (1) |
GPL7294 |
Agilent-014879 Whole Rat Genome Microarray 4x44K G4131F (Probe Name version) |
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Samples (21)
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GSM1225999 |
Lung_Control, 7 days |
GSM1226000 |
Lung_L-SWCNT, 7 days |
GSM1226001 |
Lung_H-SWCNT, 7 days |
GSM1226002 |
Lung_Control, 30 days |
GSM1226003 |
Lung_L-SWCNT, 30 days |
GSM1226004 |
Lung_H-SWCNT, 30 days |
GSM1226005 |
Lung_Control, 90 days |
GSM1226006 |
Lung_L-SWCNT, 90 days |
GSM1226007 |
Lung_H-SWCNT, 90 days |
GSM1226008 |
Lung_Control, 180 days |
GSM1226009 |
Lung_L-SWCNT, 180 days |
GSM1226010 |
Lung_H-SWCNT, 180 days |
GSM1226011 |
Lung_Control, 365 days |
GSM1226012 |
Lung_L-SWCNT, 365 days |
GSM1226013 |
Lung_H-SWCNT, 365 days |
GSM1226014 |
Lung_Control, 754 days |
GSM1226015 |
Lung_L-SWCNT, 754 days |
GSM1226016 |
Lung_H-SWCNT, 754 days |
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Relations |
BioProject |
PRJNA218458 |
Supplementary file |
Size |
Download |
File type/resource |
GSE50664_RAW.tar |
719.5 Mb |
(http)(custom) |
TAR (of TXT) |
Processed data included within Sample table |
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