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Series GSE50664 Query DataSets for GSE50664
Status Public on Sep 07, 2013
Title Gene expression profiles in rat lung following intratracheal instillation with single-wall carbon nanotubes
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary Gene expression profiling of the rat lung following intratracheal instillation with single-wall carbon nanotubes (SWCNTs) was employed to gain insights into these molecular events. We attempted to characterize time-dependent changes in the gene expression until 754 days after intratracheal instillation with SWCNTs suspensions at 0.2 mg (L-SWCNT) and 0.4 mg (H-SWCNT) injected dose per rat, and to identify the shift from the acute-phase to the chronic-phase phase on the basis of evaluation at the molecular level.
 
Overall design Groups of nine-week-old male Wistar rats (n= 6 per group/ time point) were intratracheally instilled with single-wall carbon nanotubes (SWCNTs) suspended in 0.4 ml distilled water including 0.1% Triton X-100 as a single injection 0.1 mg (L-SWCNT) and 0.4 mg (H-SWCNT) SWCNTs/ rat). Control groups received 0.1% Triton X-100 (vehicle control). After intratracheal instillation treatment, rats were housed within polycarbonate cages at a controlled temperature of 22 °C with a chow diet ad libitum, and dissected at 3 days, 7 days, 30 days, 90 days, 180 days, 365 days and 754 days post-instillation. Right lungs of anesthetized rats were perfused with physiological saline, excised, and used for DNA microarray analysis.
 
Contributor(s) Fujita K, Fukuda M
Citation(s) 24911292
Submission date Sep 06, 2013
Last update date Sep 15, 2014
Contact name Katsuhide Fujita
E-mail(s) ka-fujita@aist.go.jp
Organization name National Institute of Advanced Industrial Science and Technology
Department Research Institute of Science for Safety and Sustainability
Street address Onogawa 16-1
City Tsukuba
ZIP/Postal code 3058569
Country Japan
 
Platforms (1)
GPL7294 Agilent-014879 Whole Rat Genome Microarray 4x44K G4131F (Probe Name version)
Samples (21)
GSM1225996 Lung_Control, 3 days
GSM1225997 Lung_L-SWCNT, 3 days
GSM1225998 Lung_H-SWCNT, 3 days
Relations
BioProject PRJNA218458

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE50664_RAW.tar 719.5 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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