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Series GSE50672 Query DataSets for GSE50672
Status Public on Jan 01, 2014
Title Ablation of coactivator Med1 regulates bulge keratinocyte stem cells and accelerates epidermal regeneration after injury [10wk keratinocyte]
Organism Mus musculus
Experiment type Expression profiling by array
Summary Transcriptional coactivator Mediator complex facilitates transcription of various transcription factors. Previously, we have generated Med1 conditional null mice, where a critical subunit of Mediator, Med1, is removed from keratinocytes. Here we present evidence that ablation of Med1 accelerated epidermal regeneration after injury. As bulge keratinocyte stem cells are important contributors to regenerate epidermis, we first analyzed properties of stem cells in Med1 null mice. BrdU long retaining analysis revealed that deletion of Med1 still maintained quiescence of bulge keratinocyte stem cells, despite of general hyperplasia observed in Med1 deficient keratinocytes. Gene expression analysis demonstrated that a series of niche matrix proteins decreased in Med1 deficient keratinocytes. In contrast, the expression of stem cell marker Sox9 was not altered, suggesting stem cells are present but activated because of abnormal niche surrounding stem cells. In addition, Med1 deletion suppressed injury induced inflammatory reaction, which indirectly regulates epidermal regeneration. We also indicated that TGFβ1 significantly decreased in both bulge and epidermal keratinocytes upon Med1 deletion. Our study demonstrates that coactivator Med1 has a critical role to maintain bulge stem cells and epidermal regeneration presumably through regulation in TGFβ signaling.
 
Overall design n=3 WT and KO (each group contains keratinocytes isolated from adult skins excised from 2 mice)
 
Contributor(s) Hu L, Nguyen T, Fong FC, Bikle DD, Oda Y
Citation(s) 32857768
Submission date Sep 06, 2013
Last update date Sep 02, 2020
Contact name Yuko Oda
E-mail(s) yuko.oda@ucsf.edu
Phone 4152692370
Organization name VA Medical Center UCSF
Department Endocrine Research
Street address 111N-MB 360 1700 Owens St, Suite 360
City San Francisco
State/province CA
ZIP/Postal code 94158
Country USA
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (6)
GSM1226054 Control (WT) keratinocyte rep1
GSM1226055 Control (WT) keratinocyte rep2
GSM1226056 Control (WT) keratinocyte rep3
This SubSeries is part of SuperSeries:
GSE50673 Ablation of coactivator Med1 regulates bulge keratinocyte stem cells and accelerates epidermal regeneration after injury.
Relations
BioProject PRJNA218223

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE50672_RAW.tar 3.1 Mb (http)(custom) TAR
GSE50672_non_normalized.txt.gz 678.0 Kb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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