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Series GSE50678 Query DataSets for GSE50678
Status Public on Dec 31, 2013
Title Transcriptional Response to transgenic BRAF(V600K) in the intestinal epithelium
Organism Mus musculus
Experiment type Expression profiling by array
Summary We have analysed transcriptional effects of BRAF(V600K) in the intestinal epithelium of transgenic mice that harbour an inducible BRAF(V600K) transgene.
Mice used in this experiment were compound transgenic for a stem-cell specific Lgr5-EGFP Reporter and either an inducible TdTomato-2A-BRAF(V600K) transgene or an inducibe TdTomato-luciferase transgene.
 
Overall design Transgenes were induced by doxycycline (4mg/ml) treatment provided in a 1% sucrose solution in the drinking water of transgenic mice for 24h. Intestinal crypts were isolated by filtering (70um) following a PBS/EDTA incubation step. Single cell suspensions were made by digestion of crypts in 500ug/ml trypsine and 0.8u/ml DNAseI for approx. 20min at RT. Cell populations for profiling were isolated by FACS, using an Aria SOPR (BD), equipped with a 70um nozzle.
 
Contributor(s) Morkel M, Riemer P
Citation(s) 25109331
Submission date Sep 06, 2013
Last update date Jun 14, 2018
Contact name Markus Morkel
E-mail(s) markus.morkel@charite.de
Organization name Charité - Universitätsmedizin Berlin
Department Dept. of Pathology
Lab Laboratory of Molecular Tumour Pathology
Street address Charitéplatz 1
City Berlin
ZIP/Postal code 10117
Country Germany
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (16)
GSM1226197 luc_control-G-rep1
GSM1226198 luc_control-RG-rep1
GSM1226199 luc_control-G-rep2
Relations
BioProject PRJNA218462

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE50678_RAW.tar 3.1 Mb (http)(custom) TAR
GSE50678_non-normalized.txt.gz 1.7 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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