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Series GSE50756 Query DataSets for GSE50756
Status Public on Sep 12, 2013
Title Cyclophilin B supports the survuval of glioblastoma multiforme cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary We have found that cyclophilin B (CypB) expression is important for malignant glioblastoma multiforme (GBM) cell proliferation. To identify molecular mechanisms that could explain CypB-dependent survival in human GBM cells, a microarray analysis was performed using RNA prepared from U251MG GBM cells transduced with lentiviral CypB shRNA. These data revealed that about 130 genes were more than 2-fold affected by CypB depletion. Significant alterations in the expression of genes related to cell death, cell proliferation and cell migration were found in the shCypB cells.
Overall design U251 glioblastoma cells transduced with lentivirus expressing non-target control shRNA (shCON) were compared to cells transduced with lentivirus expressing shRNA sequence against cyclophilin B (shCypB). Cells transduced with the lentiviral shRNA (shCON VS shCypB) for 5 days before total RNA extraction. Three biological replicates of cells treated with each shRNA (shCON or shCypB) were profiled.
Contributor(s) Bram RJ, Choi JW
Citation(s) 24272483
Submission date Sep 11, 2013
Last update date Aug 13, 2018
Contact name Richard J Bram
Organization name Mayo Clinic
Department Pediatric and Adolescent Medicine
Street address 200 First Street SW
State/province MN
ZIP/Postal code 55905
Country USA
Platforms (1)
GPL10558 Illumina HumanHT-12 V4.0 expression beadchip
Samples (6)
GSM1228046 shCON1
GSM1228047 shCON2
GSM1228048 shCON3
BioProject PRJNA218845

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE50756_RAW.tar 26.2 Mb (http)(custom) TAR
GSE50756_non-normalized.txt.gz 1.9 Mb (ftp)(http) TXT
Processed data included within Sample table
Raw data are available on Series record

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