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Series GSE50818 Query DataSets for GSE50818
Status Public on Sep 11, 2014
Title Peg3 mutational effects on reproduction and placenta-specific gene families
Organism Mus musculus
Experiment type Expression profiling by array
Summary Peg3 (paternally expressed gene 3) is an imprinted gene encoding a DNA-binding protein. This gene plays important roles in controlling fetal growth rates and nurturing behaviors. In the current study, a new mutant mouse model has been generated to further characterize the functions of this DNA-binding protein. Besides known phenotypes, this new mutant model also revealed potential roles of Peg3 in mammalian reproduction. Female heterozygotes produce a much smaller number of mature oocytes than the wild-type littermates, resulting in reduced litter sizes. According to genome-wide expression analyses, several placenta-specific gene families are de-repressed in the brain of Peg3 heterozygous embryos, including prolactin, cathepsin and carcinoembryonic antigen cell adhesion molecule (Ceacam) families. The observed de-repression is more pronounced in females than in males. The de-repression of several members of these gene families is observed even in the adult brain, suggesting potential defects in epigenetic setting of the placenta-specific gene families in the Peg3 mutants. Overall, these results indicate that Peg3 likely controls the transcription of several placenta-specific gene families, and further suggest that this predicted transcriptional control by Peg3 might be mediated through unknown epigenetic mechanisms.
 
Overall design Mouse Breeding Two different types of heterozygotes were prepared for breeding experiment: male and female heterozygotes carrying the paternally (+/-p) and maternally (-m/+) transmitted targeted allele. These 4 heterozygotes were bred with their littermates: for Breeding 1, female wild-type littermates x male heterozygotes (+/-P); Breeding 2, female heterozygotes (+/-P) x male wild-type littermates; Breeding 3, female wild-type littermates x male heterozygotes (-m/+); for Breeding 4, female heterozygotes (-m/+) x male wild-type littermates. Genome-wide expression analysis A litter of 14.5-dpc fetuses were harvested from a timed mating between a male heterozygote (-m/+) and wild-type female littermate. Three tissues were obtained from each fetus: embryo head, placenta and amnionic sac. Total 16 samples were used for this series of genome-wide expression analyses: 8 embryo heads and placenta representing two biological replicates of each of the four following combinations: male wild-type, male heterozygote, female wild-type, and female heterozygote.
 
Contributor(s) Kim J, Frey WD, He H, Kim H, Ekram MB, Bakshi A, Faisal M, Perera BU, Ye A, Teruyama R
Citation(s) 24391757
Submission date Sep 12, 2013
Last update date Aug 08, 2019
Contact name Joomyeong Kim
E-mail(s) jkim@lsu.edu
Phone 225-578-7692
Organization name Louisiana State University
Department Biological Sciences
Street address 202 Life Sciences
City Baton Rouge
State/province LA
ZIP/Postal code 70803
Country USA
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (16)
GSM1230325 Placenta_wild type_female_replicate1
GSM1230326 Placenta_knockout_male_replicate1
GSM1230327 Placenta_knockout_female_replicate1
Relations
BioProject PRJNA219071

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE50818_RAW.tar 3.1 Mb (http)(custom) TAR
GSE50818_non-normalized.txt.gz 2.6 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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